Müller H, Böhm K J, Vater W, Unger E
Central Institute of Microbiology and Experimental Therapy, Academy of Sciences of the GDR, Jena.
Acta Histochem Suppl. 1990;39:373-8.
By means of electron microscopy we have investigated the influence of dilution and cold incubation (0 degree C) on mixed populations of tubulin assemblies consisting of microtubules (MTs) and protofilament ribbons with C- and S-shaped profiles formed in the presence of glycerol. Dilution results in a partial disappearance of ribbons, whereas cold incubation causes a decrease of the percentage of MTs in favour of C-ribbons, probably produced by splitting of MT ends. In the case of dilution, we assume a lower dynamic stability of ribbons compared with MTs, which was already observed during long-time incubation of mixed population (Böhm et al., Biochem. Biophys. Acta 929, 154, 1987), whereas the splitting effect in the cold should be caused by the deficiency of MT-bound microtubule-associated proteins (MAPs), which is observed in the presence of glycerol.
通过电子显微镜，我们研究了稀释和冷孵育（0摄氏度）对由微管（MTs）和原纤维带组成的微管蛋白组装混合群体的影响，这些原纤维带在甘油存在的情况下形成了C形和S形轮廓。稀释导致带部分消失，而冷孵育导致MTs百分比下降，有利于C带，这可能是由MT末端分裂产生的。在稀释的情况下，我们假设与MTs相比，带的动态稳定性较低，这在混合群体的长时间孵育过程中已经观察到（Böhm等人，《生物化学与生物物理学报》929, 154, 1987），而冷中的分裂效应应该是由与MT结合的微管相关蛋白（MAPs）缺乏引起的，这在甘油存在的情况下可以观察到。
