Linsel G, Zglinicki T
Department of Ultrastructural Pathology and Electron Microscopy, Central Institute of Occupational Medicine of the GDR, Berlin.
Acta Histochem Suppl. 1990;39:485-8.
The lement concentrations of intracellular compartments of leaf cells of Egeria densa are estimated by X-ray microanalysis. Ultrathin frozen-dried sections are used in TEM mode. The concentrations of Na, Mg, P, Cl, K, and Ca are determined in the cell wall, cytoplasm and chloroplasts. The cell wall represents a Donnan free space with a negative fixed charge concentration of 360 mval/l water. The water fraction of the cytoplasm is between 85 and 90% and strikes the bounds of possibility of that method.
通过X射线微分析估算了水蕴草叶片细胞内区室的元素浓度。在透射电子显微镜（TEM）模式下使用超薄冷冻干燥切片。测定了细胞壁、细胞质和叶绿体中钠、镁、磷、氯、钾和钙的浓度。细胞壁代表一个唐南自由空间，其固定负电荷浓度为360毫伏/升水。细胞质的水分含量在85%至90%之间，达到了该方法可能性的极限。
