State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
Appl Microbiol Biotechnol. 2011 Jan;89(1):57-62. doi: 10.1007/s00253-010-2853-3. Epub 2010 Aug 28.
1,3-Propanediol is an important chemical widely used in polymer production, but its availability is being restricted owing to its expensive synthesis. The aim of this study was to engineer an Escherichia coli strain that can produce 1,3-propanediol directly from glucose. We successfully constructed a stress-induced metabolic pathway from glucose to 1,3-propanediol in recombinant E. coli by the expression of gpd1 and gpp2 genes from Saccharomyces cerevisiae and dha operon from Klebsiella pneumoniae, respectively. Batch cultivation of the recombinant E. coli showed that 12.1 g/L 1,3-propanediol was accumulated in the culture without using any inducer.
1,3-丙二醇是一种重要的化学物质，广泛应用于聚合物生产中，但由于其昂贵的合成方法，其可用性受到限制。本研究的目的是构建一株能够直接从葡萄糖生产 1,3-丙二醇的大肠杆菌菌株。我们通过表达来自酿酒酵母的 gpd1 和 gpp2 基因以及来自肺炎克雷伯氏菌的 dha 操纵子，成功地在重组大肠杆菌中构建了一条从葡萄糖到 1,3-丙二醇的应激诱导代谢途径。重组大肠杆菌的分批培养表明，在不使用任何诱导剂的情况下，培养物中积累了 12.1 g/L 的 1,3-丙二醇。
