CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, Beijing, P R China.
Electrophoresis. 2010 Sep;31(18):3144-51. doi: 10.1002/elps.201000133.
In this report we fabricated laminin (LN) stripes on a background of poly-L-lysine as substrates for the growth of rat hippocampal neurons, and found that a sharp change of the concentration of LN guides the growth of neurites by leading the growth cones in a time- and space-dependent manner. The percentage of neurites that grow along the edge of LN stripes (where there is a sharp change of concentration) decreases as a function of the concentration of LN under a threshold value. The actin cytoskeleton plays an important role in the process of growth cone's response to the sharp change of concentration of LN on micropatterns. We believe that the findings here are useful for not only fundamental studies in neuroscience, but also helpful for the design of devices or chips for nervous prosthesis.
在本报告中，我们在聚-L-赖氨酸背景上制造了层粘连蛋白 (LN) 条纹作为大鼠海马神经元生长的底物，并发现 LN 浓度的急剧变化通过时间和空间依赖的方式引导生长锥，从而指导突起的生长。沿着 LN 条纹（浓度急剧变化的地方）生长的突起的百分比随着 LN 浓度低于阈值时的浓度的函数而降低。肌动蛋白细胞骨架在生长锥对微图案上 LN 浓度急剧变化的反应过程中起着重要作用。我们相信，这里的发现不仅对神经科学的基础研究有用，而且对神经假体的器件或芯片的设计也有帮助。
