Erben R G, Kohn B, Rambeck W A, Zucker H
Institute of Physiology, Physiological Chemistry and Nutrition Physiology, Veterinary Faculty, Ludwig-Maximilians-University, München, Federal Republic of Germany.
Scanning Microsc. 1990 Sep;4(3):625-38; discussion 638-40.
Twenty-four female Sprague-Dawley rats (10 weeks old, 200g BW) were either sham-operated (n = 6) or ovariectomized (ovx). Ovx rats were divided into 3 groups (n = 6 each): ovx; ovx + 1,25-(OH)2D3; ovx + 1,25(OH)2D3 + 1,24,25-(OH)3D3. The vitamin D metabolites were fed orally starting the day after surgery. After 7 weeks all rats were sacrificed and the proximal tibiae were processed undecalcified for quantitative histomorphometry. Conventional histomorphometric analysis of the distal zone (greater than 1 mm from the growth cartilage) of the tibial metaphysis revealed a dramatic loss of cancellous bone mass in ovx rats. Both 1,25(OH)2D3 and the combination of 1,25(OH)2D3 with 1,24,25(OH)3D3 prevented the bone loss in the distal zone in ovx animals. Measurements in the proximal zone (less than 1 mm from the growth cartilage) of the tibial metaphysis were performed with a newly developed technique that utilizes the advantages of automatic image analysis, and that we propose to name "linear scanning". This method revealed a significantly decreased hard tissue mass at about 100 microns and within 800 to 950 microns distance from the growth plate in ovx rats. However, ovx rats reached normal amounts of hard tissue within 250 to 450 microns from the growth plate. The results obtained by linear scanning suggest that the obvious loss of cancellous bone mass in the distal zone of the tibial metaphysis in growing ovx rats is not a consequence of structural changes in the proximal zone.
选用24只10周龄、体重200克的雌性Sprague-Dawley大鼠，其中6只进行假手术，其余大鼠行卵巢切除术（ovx）。卵巢切除的大鼠分为3组（每组6只）：ovx组；ovx + 1,25-(OH)2D3组；ovx + 1,25(OH)2D3 + 1,24,25-(OH)3D3组。术后次日开始经口给予维生素D代谢物。7周后处死所有大鼠，取近端胫骨进行不脱钙处理，用于定量组织形态计量学分析。对胫骨近端干骺端远侧区（距生长软骨大于1毫米）进行常规组织形态计量学分析发现，卵巢切除大鼠的松质骨量显著减少。1,25(OH)2D3以及1,25(OH)2D3与1,24,25(OH)3D3联合使用均可防止卵巢切除动物远侧区的骨质流失。采用一种新开发的技术对胫骨近端干骺端近侧区（距生长软骨小于1毫米）进行测量，该技术利用了自动图像分析的优势，我们建议将其命名为“线性扫描”。该方法显示，卵巢切除大鼠在距生长板约100微米以及800至950微米范围内的硬组织量显著减少。然而，卵巢切除大鼠在距生长板250至450微米范围内的硬组织量达到正常水平。线性扫描结果表明，生长中的卵巢切除大鼠胫骨近端干骺端远侧区松质骨量的明显减少并非近侧区结构变化的结果。
