曼氏血吸虫：甲氟喹和伯氨喹体外杀血吸虫效果。
Schistosoma mansoni: schistosomicidal effect of mefloquine and primaquine in vitro.
机构信息
Division of Tropical Medicine and Infectious Diseases, Department of Internal Medicine, University of Rostock, Germany.
出版信息
Exp Parasitol. 2011 Jan;127(1):270-6. doi: 10.1016/j.exppara.2010.08.008. Epub 2010 Aug 31.
We investigated the effects of the anti-malarials mefloquine and primaquine against the juvenile and adult life stages of Schistosoma mansoniin vitro. Cercariae were incubated with 0.5 μg/ml, 1 μg/ml and 2 μg/ml mefloquine or primaquine and with 1 μg/ml praziquantel for 12h. Schistosomula, pre-adults and adults were incubated with 0.5 μg/ml, 1 μg/ml and 2 μg/ml mefloquine or primaquine and with 1 μg/ml praziquantel for 7 days. The viability status was classified as viable, damaged or dead and was checked every 3h for cercariae and every 12h for schistosomula, pre-adults and adults. Both, mefloquine and primaquine show time and dose-dependent schistosomicidal effects on the four life stages of S. mansoni. The promising in vitro effects on all stages of the blood fluke S. mansoni warrants further evaluation of both anti-malarials and their derivatives for their prophylactic and therapeutic values in early and late schistosomiasis in field trials.
我们研究了抗疟药甲氟喹和伯氨喹对曼氏血吸虫幼体和成虫期的体外影响。用 0.5μg/ml、1μg/ml 和 2μg/ml 的甲氟喹或伯氨喹以及 1μg/ml 的吡喹酮孵育尾蚴 12 小时。用 0.5μg/ml、1μg/ml 和 2μg/ml 的甲氟喹或伯氨喹以及 1μg/ml 的吡喹酮孵育 7 天，检测童虫、未成熟和成虫的活力状态，分为存活、受损或死亡，并每 3 小时检查一次尾蚴，每 12 小时检查一次童虫、未成熟和成虫。甲氟喹和伯氨喹对曼氏血吸虫的四个生活阶段均表现出时间和剂量依赖性的杀血吸虫效果。这两种抗疟药及其衍生物在现场试验中对预防和治疗早期和晚期血吸虫病具有潜在的应用价值，值得进一步评估其在所有血吸虫阶段的应用。
Zotero 插件