The in Vitro Antischistosomal Activity and Genotoxicity of the Active Ingredients of (allicin) and (curcumin).

BACKGROUND

In this study, we assessed the in vitro antischistosomal activity of the active ingredients of (allicin) and (curcumin) on .

METHODS

This study was conducted in Faculty of Science, Port said University, Egypt (2018). Adult worms were exposed to a range of concentrations of AL or CU, and worm survival was assessed 24 h post-exposure to calculate the lethal concentration of the compounds. Scanning electron microscopy was used to assess ultrastructural changes in the surface of AL- or CU- treated worms. The genotoxicities of AL and CU on were determined by DNA fragmentation analysis.

RESULTS

We determined the concentrations of AL and CU required to kill 50% of (LC ). The LC of AL was 8.66 μL/mL, whereas 100% mortality of was achieved by AL at concentrations of 50 μL/mL. The LC of CU was 87.25 μL/mL, with the highest mortality of 91.3% seen after 24 h exposure to 100 μg/mL CU. Ultrastructural studies revealed that exposure to either AL or CU led to mild or severe surface damage to , respectively. The degree of damage in the worms was sex-dependent. Interestingly, while CU exposure resulted in DNA fragmentation in worms, we observed no genotoxic effects of AL.

CONCLUSION

Both AL and CU exhibit antischistosomal activity; the study provided evidence suggesting that these compounds act through distinct mechanisms. These promising results encourage further investigation into these compounds as potential antischistosomal agents, either alone or as complementary treatments to praziquantel.