Rashed Hadeer Abd El-Hak, Abu Almaaty Ali Hussein, Soliman Maha Farid Mohamed, El-Shenawy Nahla Soliman
Zoology Department, Faculty of Science, Port Said University, Port Said, Egypt.
Zoology Department, Faculty of Science, Suez Canal University, Ismailia, 41522, Egypt.
Iran J Parasitol. 2021 Jan-Mar;16(1):101-110. doi: 10.18502/ijpa.v16i1.5540.
In this study, we assessed the in vitro antischistosomal activity of the active ingredients of (allicin) and (curcumin) on .
This study was conducted in Faculty of Science, Port said University, Egypt (2018). Adult worms were exposed to a range of concentrations of AL or CU, and worm survival was assessed 24 h post-exposure to calculate the lethal concentration of the compounds. Scanning electron microscopy was used to assess ultrastructural changes in the surface of AL- or CU- treated worms. The genotoxicities of AL and CU on were determined by DNA fragmentation analysis.
We determined the concentrations of AL and CU required to kill 50% of (LC ). The LC of AL was 8.66 μL/mL, whereas 100% mortality of was achieved by AL at concentrations of 50 μL/mL. The LC of CU was 87.25 μL/mL, with the highest mortality of 91.3% seen after 24 h exposure to 100 μg/mL CU. Ultrastructural studies revealed that exposure to either AL or CU led to mild or severe surface damage to , respectively. The degree of damage in the worms was sex-dependent. Interestingly, while CU exposure resulted in DNA fragmentation in worms, we observed no genotoxic effects of AL.
Both AL and CU exhibit antischistosomal activity; the study provided evidence suggesting that these compounds act through distinct mechanisms. These promising results encourage further investigation into these compounds as potential antischistosomal agents, either alone or as complementary treatments to praziquantel.
在本研究中，我们评估了大蒜素和姜黄素的活性成分对埃及血吸虫的体外抗血吸虫活性。
本研究于2018年在埃及塞得港大学理学院进行。将成虫暴露于一系列浓度的大蒜素或姜黄素中，并在暴露24小时后评估虫体存活率，以计算化合物的致死浓度。使用扫描电子显微镜评估经大蒜素或姜黄素处理的虫体表面的超微结构变化。通过DNA片段化分析确定大蒜素和姜黄素对埃及血吸虫的遗传毒性。
我们确定了杀死50%埃及血吸虫所需的大蒜素和姜黄素浓度（半数致死浓度）。大蒜素的半数致死浓度为8.66μL/mL，而在50μL/mL浓度下大蒜素可使埃及血吸虫100%死亡。姜黄素的半数致死浓度为87.25μL/mL，在暴露于100μg/mL姜黄素24小时后，最高死亡率为91.3%。超微结构研究表明，暴露于大蒜素或姜黄素分别导致埃及血吸虫表面轻度或严重损伤。虫体的损伤程度与性别有关。有趣的是，虽然暴露于姜黄素会导致埃及血吸虫虫体DNA片段化，但我们未观察到大蒜素有遗传毒性作用。
大蒜素和姜黄素均表现出抗血吸虫活性；该研究提供的证据表明，这些化合物通过不同的机制发挥作用。这些有前景的结果鼓励进一步研究这些化合物作为潜在的抗血吸虫药物，单独使用或作为吡喹酮的辅助治疗。
