Weichert H, Lehmann J, Ambrosius H
Karl-Marx University, Section of Biosciences, Leipzig.
Biomed Biochim Acta. 1990;49(10):999-1004.
The cultivation and stimulation of human peripheral blood cells by different mitogens were studied using a serum-free culture technique. It shows the possibility to culture human PBL in a commercially available serum-free medium over a period of at least 7 days. In a serum-free medium with high protein content the viability of the cells was higher than in one with low protein content or in a serum containing medium. It is possible to stimulate human T cells by phytohaemagglutinin in a serum-free medium. After measurement of [3H]thymidine incorporation, a significant difference between control and test cultures was found. There is also a difference between serum-free medium with high and low protein content. Human peripheral B lymphocytes stimulated by pokeweed mitogen did not show any measurable IgG-secretion if they were cultured in serum-free medium.
采用无血清培养技术研究了不同促细胞分裂剂对人外周血细胞的培养和刺激作用。结果表明，在市售的无血清培养基中培养人外周血淋巴细胞至少7天是可行的。在高蛋白含量的无血清培养基中，细胞的活力高于低蛋白含量的无血清培养基或含血清的培养基。在无血清培养基中，植物血凝素能够刺激人T细胞。在测定[3H]胸腺嘧啶核苷掺入量后，发现对照培养物和试验培养物之间存在显著差异。高蛋白含量和低蛋白含量的无血清培养基之间也存在差异。如果在无血清培养基中培养，由商陆促细胞分裂剂刺激的人外周B淋巴细胞不会分泌任何可检测到的IgG。
