Shankey T V, Daniele R P, Nowell P C
Clin Exp Immunol. 1981 Apr;44(1):156-66.
Using a culture system that includes a B-enriched fraction of peripheral blood lymphocytes (PBL) mixed with non-proliferating "filler" cells (mitomycin-treated PBL or T cells), the kinetics of human B cell proliferation and differentiation into cells containing cytoplasmic immunoglobulin (cIg) have studied. Following stimulation with phytohaemagglutinin (PHA) or pokeweed mitogen (PWM), proliferative responses of enriched B cells were comparable to those for unseparated PBL, with a peak at 3 to 5 days and a steep decline thereafter; cIg-positive cells increased with both mitogens from day 3 until 7. Little proliferation or differentiation occurred in B cell cultures lacking T "filler" cells. The results indicate that human B cells can respond polyclonally to PHA in vitro, as well as to PWM, if non-proliferating T cell "help" is present. Further, these proliferative responses, but not subsequent differentiation, were inhibited by PHA-stimulated blasts that were treated with mitomycin and added on day 3 of culture.
利用一种培养系统，该系统包括富含B细胞的外周血淋巴细胞（PBL）部分与非增殖性“填充”细胞（丝裂霉素处理的PBL或T细胞）混合，研究了人B细胞增殖以及分化为含有细胞质免疫球蛋白（cIg）细胞的动力学。在用植物血凝素（PHA）或商陆有丝分裂原（PWM）刺激后，富集的B细胞的增殖反应与未分离的PBL的增殖反应相当，在3至5天达到峰值，此后急剧下降；cIg阳性细胞在两种有丝分裂原刺激下从第3天到第7天均增加。在缺乏T“填充”细胞的B细胞培养物中几乎没有增殖或分化发生。结果表明，如果存在非增殖性T细胞“辅助”，人B细胞在体外可对PHA以及PWM产生多克隆反应。此外，这些增殖反应，但不是随后的分化，受到在培养第3天添加的经丝裂霉素处理的PHA刺激的母细胞的抑制。
