Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture/Hi-Tech Key Laboratory of Information Agriculture of Jiangsu Province, Nanjing Agricultural University, Nanjing, China.
Biol Trace Elem Res. 2011 Sep;142(3):807-18. doi: 10.1007/s12011-010-8812-0. Epub 2010 Sep 1.
In order to identify the variation of cadmium (Cd) tolerance and accumulation in wheat (Triticum aestivum L.), a study was conducted in hydroponic culture with or without Cd using recombinant inbred lines (RILs) consisting of 103 RILs derived from a cross of Chuan 35050 × Shannong 483 at seedling stage. The parameters of shoot height, secondary roots numbers, tiller numbers, shoot dry weights, root dry weights, and maximum efficiency of photosystem II photochemistry under dark-adopted conditions were measured. Cd-tolerant indexes were then calculated as relative the above traits under Cd stress to those under the control. Cd concentration in shoot or root was determined and Cd accumulation and translocation were calculated. Based on the Cd-tolerant indexes, membership function analysis was used to determine the variation of the above parameters. The results showed a continuous distribution among the RILs and then the RILs were divided into five groups according to their tolerance. Lines 76 and 17 were considered as the most Cd-tolerant lines while lines 103 and 51 were as the most Cd-sensitive lines. Meanwhile, lines 38 and 79 were with minimum Cd translocation ratio while lines 88 and 53 were with maximum Cd translocation ratio, respectively. The relationship between Cd tolerance and accumulation was not significant, indicating Cd tolerance and accumulation may be independent traits in the RILs. Thus, lines with high Cd tolerance and less Cd accumulation could be selected for wheat breeding.
为了鉴定小麦（Triticum aestivum L.）对镉（Cd）耐性和积累的变异，在水培条件下进行了一项研究，使用由川 35050×山农 483 杂交后代的 103 个重组自交系（RILs）进行了有或无 Cd 的处理。测量了幼苗期的株高、次生根数量、分蘖数、地上部干重、根干重和暗适应条件下最大光系统 II 光化学效率等参数。然后，根据 Cd 胁迫下的上述性状相对于对照下的性状，计算 Cd 耐性指数。测定地上部或根部的 Cd 浓度，并计算 Cd 积累和转运。基于 Cd 耐性指数，使用隶属函数分析来确定上述参数的变异。结果表明，RILs 之间存在连续分布，然后根据其耐性将 RILs 分为五组。76 号和 17 号线被认为是最耐 Cd 的线，而 103 号和 51 号线是最敏感的 Cd 线。同时，38 号和 79 号线的 Cd 转运比率最小，而 88 号和 53 号线的 Cd 转运比率最大。Cd 耐性和积累之间的关系不显著，表明 Cd 耐性和积累可能是 RILs 中的独立性状。因此，可以选择具有高 Cd 耐性和低 Cd 积累的系进行小麦育种。
