荧光原位杂交（FISH），基本原理与方法。

Fluorescence in situ hybridization (FISH), basic principles and methodology.

作者信息

Garimberti Elisa, Tosi Sabrina

机构信息

Laboratory of Leukaemia and Chromosome Research, Division of Biosciences, Centre for Cell & Chromosome Biology, Brunel Institute for Cancer Genetics and Pharmacogenomics, Brunel University, Uxbridge, Middlesex, UK.

出版信息

Methods Mol Biol. 2010;659:3-20. doi: 10.1007/978-1-60761-789-1_1.

DOI:10.1007/978-1-60761-789-1_1

PMID:20809300

Abstract

Fluorescence in situ hybridization (FISH) is widely used for the localization of genes and specific genomic regions on target chromosomes, both in metaphase and interphase cells. The applications of FISH are not limited to gene mapping or the study of genetic rearrangements in human diseases. Indeed, FISH is increasingly used to explore the genome organization in various organisms and extends to the study of animal and plant biology. We have described the principles and basic methodology of FISH to be applied to the study of metaphase and interphase chromosomes.

摘要

荧光原位杂交（FISH）广泛用于在中期和间期细胞的目标染色体上定位基因和特定基因组区域。FISH的应用不仅限于基因图谱绘制或人类疾病中基因重排的研究。实际上，FISH越来越多地用于探索各种生物体中的基因组组织，并扩展到动植物生物学的研究。我们已经描述了应用于中期和间期染色体研究的FISH的原理和基本方法。