Soloviev I V, Vorsanova S G, Malet P, Zerova T E, Buzhievskaya T I
National Research Center of Mental Health, RAMS, Moscow.
Tsitol Genet. 1998 Jul-Aug;32(4):60-4.
Double color in situ hybridization was used to determine the specificity of cloned alpha-satellite cosmid DNA sequences belonging to satellite DNA sequences shared by chromosomes 13 and 21. It was determined that cosmid clone cos56D8 is more specific to chromosome 13, cosmid clone cos37E is more specific to chromosome 21 and cosmid clones cosA5130 is specific to both chromosomes 13 and 21. These new cosmid probes could bw useful in the molecular-cytogenetic analysis of trisomic cells at Patau or Down syndromes as well as in analysis of marker chromosomes originated from the chromosomes 13 and 21.
采用双色原位杂交技术来确定属于13号和21号染色体共有的卫星DNA序列的克隆α-卫星黏粒DNA序列的特异性。已确定黏粒克隆cos56D8对13号染色体更具特异性，黏粒克隆cos37E对21号染色体更具特异性，而黏粒克隆cosA5130对13号和21号染色体均具有特异性。这些新的黏粒探针可用于帕陶氏综合征或唐氏综合征三体细胞的分子细胞遗传学分析，以及源自13号和21号染色体的标记染色体的分析。
