The in vitro toxic response of spiropyrans in cellular models has not been previously addressed, despite the fact that such photoswitchable molecules have shown great potential as versatile and tunable components for bionanodevices and imaging agents. In this study, we examine the cytotoxic effects of a spiropyran, namely, 8-methoxy-6-nitro-BIPS (1',3'-dihydro-1'-ethanol-3',3'-dimethyl-8-methoxy-6-nitro-spiro(2H-1-benzopyran-2,2'-(2H)-indole) [1], in three cultured cellular models (THP-1, AGS, and A549 cell lines) by High Content Screening and Analysis (HCSA) and by enzyme-linked immunosorbent (ELISA) assays (Interleukin-6 and Tumor Necrosis Factor-alpha). The HCSA results show that low concentrations of 8-methoxy-6-nitro-BIPS (10(-6), 10(-8), and 10(-9) M) do not induce any cytotoxic response after 24 and 72 h exposure time, while at the highest concentrations (10(-3)and 10(-4) M) the exposure time becomes a critical parameter of the toxic response. The cell viability is reduced by 60% for THP-1 cells, 50% for AGS cells, and 40% for A549 cells at a spiropyran concentration of 10(-3) M after 24 h incubation, whereas at 72 h, the cell loss increases above 90%. Interestingly, at 10(-4) M no significant cytotoxic response is registered after 24 h exposure, where contrarily cytotoxicity is verified after 72 h. Our ELISA results show that consistently with the HCSA analysis a robust inflammatory response is present at 10(-3) M after 24 h exposure and at 10(-3) M and 10(-4) M after 72 h, in all three cell lines investigated.