Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil.
J Periodontol. 2011 Jan;82(1):86-95. doi: 10.1902/jop.2010.100248. Epub 2010 Sep 1.
Chronic periodontitis (CP) and aggressive periodontitis (AP) are inflammatory diseases and the main cause of dental loss in adults. We aimed to investigate the expression of adhesion molecules and the source of proinflammatory and anti-inflammatory cytokines in circulating mononuclear cells from patients with CP and AP.
Peripheral blood mononuclear cells from healthy controls and CP or AP patients were collected. The expression of the cell adhesion molecules CD11a and CD11b, and the cellular sources of interleukin (IL)-4, IL-10, IL-12, interferon-γ, and tumor necrosis factor-α by distinct subpopulations of circulating leukocytes were determined using flow cytometry.
The expression of CD11a, but not CD11b, was significantly higher within the CD4(+) and CD8(+) T cells in CP and AP than in healthy controls. The frequencies of tumor necrosis factor-α-expressing CD4(+) T cells and CD14(+) cells were higher in AP and CP, compared to healthy controls, respectively. Moreover, the frequency of IL-10 expressing CD14(+) cells was higher in CP, but not AP, compared to healthy controls CD4(+) T cells committed to IL-4 production was higher in CP than in healthy controls.
These results suggest the participation of CD11a in the pathogenesis of periodontal lesions and show distinct cellular sources of immunoregulatory cytokines in AP versus CP.
慢性牙周炎（CP）和侵袭性牙周炎（AP）是炎症性疾病，是成年人牙齿丧失的主要原因。我们旨在研究 CP 和 AP 患者循环单核细胞中黏附分子的表达以及促炎和抗炎细胞因子的来源。
收集健康对照者和 CP 或 AP 患者的外周血单核细胞。使用流式细胞术测定循环白细胞不同亚群中细胞黏附分子 CD11a 和 CD11b 的表达以及白细胞介素（IL）-4、IL-10、IL-12、干扰素-γ和肿瘤坏死因子-α的细胞来源。
CP 和 AP 患者的 CD4(+)和 CD8(+)T 细胞中 CD11a 的表达明显高于健康对照组，但 CD11b 的表达无差异。与健康对照组相比，AP 和 CP 患者中表达肿瘤坏死因子-α的 CD4(+)T 细胞和 CD14(+)细胞的频率更高。此外，CP 患者中表达 IL-10 的 CD14(+)细胞的频率高于健康对照组，但 AP 患者中无差异。CP 患者中 CD4(+)T 细胞向 IL-4 产生的分化频率高于健康对照组。
这些结果表明 CD11a 参与牙周病的发病机制，并显示 AP 与 CP 之间抗炎细胞因子的不同细胞来源。
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