Department of Histology and Embryology, Cumhuriyet University, Faculty of Medicine, 58140 Sivas, Turkey.
Tissue Cell. 2010 Oct;42(5):301-6. doi: 10.1016/j.tice.2010.07.004.
The endometrial extracellular matrix (ECM) remodelling has a crucial role in the establishment of a successful pregnancy. In addition to its basic function such as regulation of cell function, differentiation, migration, proliferation, the substantial alterations in the endometrial ECM may play a specific role in the trophoblast invasion, placentation, cell death and formation of the proper and functional implantation chamber around the embryo. In the present study, immunolocalizations of fibronectin and laminin were determined using avidin-biotin complex-peroxidase in rat implantation sites during 7-10 days of pregnancy. Both proteins were present in the basal membrane of blood vessels and in decidual matrix whereas they were absent or had very weak reactivity in the primary decidual zone on day 7. When placentation has begun, the immunoreactivity of both proteins was increased in the placental bed and in the basal membrane of blood vessels of the mesometrial region. The immunolocalization of both proteins seemed to be decreased in the antimesometrial decidua, however, it was increased in the mesometrial decidual matrix on days 9 and 10. Therefore, it could be suggested laminin and fibronectin demonstrating dynamic expressions in relation with the morphological differentiation of endometrial stroma may play crucial roles in the control of trophoblast adhesion and invasion, in placentation and angiogenesis, in the determination of cell shape and fate thus contributing the endometrial receptivity and a successful pregnancy.
子宫内膜细胞外基质（ECM）的重塑在成功妊娠的建立中起着关键作用。除了其调节细胞功能、分化、迁移、增殖等基本功能外，子宫内膜 ECM 的实质性改变可能在滋养层细胞侵袭、胎盘形成、细胞死亡和胚胎周围适当功能植入腔的形成中发挥特定作用。在本研究中，在妊娠第 7-10 天期间，使用亲和素-生物素复合物-过氧化物酶在大鼠植入部位确定了纤连蛋白和层粘连蛋白的免疫定位。这两种蛋白均存在于血管的基膜和蜕膜基质中，而在第 7 天的初级蜕膜区，它们要么不存在，要么反应非常弱。当胎盘形成开始时，两种蛋白的免疫反应性在胎盘床和中系膜区域血管的基膜中增加。这两种蛋白的免疫定位似乎在反系膜蜕膜中减少，但在第 9 和 10 天中系膜蜕膜基质中增加。因此，可以认为纤连蛋白和层粘连蛋白的表达与子宫内膜基质的形态分化有关，可能在滋养层细胞黏附和侵袭、胎盘形成和血管生成、细胞形状和命运的决定中发挥关键作用，从而有助于子宫内膜的接受性和成功妊娠。
