Kayisli Umit A, Korgun Emin T, Akkoyunlu Gokhan, Arici Aydin, Demir Ramazan
Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, Antalya, Turkey.
Acta Histochem. 2005;107(3):173-85. doi: 10.1016/j.acthis.2005.01.005.
The reorganization of the human endometrium is termed decidualization, which includes endometrial cell proliferation, differentiation, integrin switching and extracellular matrix (ECM) remodeling during early pregnancy. The present study aimed to investigate distribution patterns, staining intensity and sex steroid-mediated regulation of integrin alpha5 (CD49e), integrin beta4 (CD49f) expression and their ligands fibronectin and laminin during decidualization. Human tissue samples were evaluated in two groups, those collected in early days and those collected in advanced days of the first trimester. Correlating immunostaining was found between laminin and integrin beta4, and between fibronectin and integrin alpha5. The expression of fibronectin was higher than that of laminin in the early days (p < 0.05). Temporal and spatial immunostaining of integrin beta4 and alpha5 in the apical pole of luminal and glandular cells was observed as pregnancy progressed (p < 0.05). In vitro results showed that human chorionic gonadotropin (hCG) stimulated laminin expression, downregulated integrin beta4 expression, whereas estradiol decreased fibronectin expression by Ishikawa cells. hCG suppressed fibronectin expression in endometrial stromal cells in culture. Our results suggest that fibronectin is responsible for induction of decidual cell differentiation, and different temporal and spatial expression of the integrins may play a role in implantation. Our in vitro results suggest that regulation of extracellular matrix remodeling and integrin switching are at least partially regulated by reproductive hormones.
人类子宫内膜的重组被称为蜕膜化，这包括妊娠早期子宫内膜细胞的增殖、分化、整合素转换和细胞外基质（ECM）重塑。本研究旨在调查蜕膜化过程中整合素α5（CD49e）、整合素β4（CD49f）及其配体纤连蛋白和层粘连蛋白的分布模式、染色强度以及性类固醇介导的调节作用。将人体组织样本分为两组进行评估，一组是在孕早期采集的，另一组是在孕早期晚期采集的。发现层粘连蛋白与整合素β4之间以及纤连蛋白与整合素α5之间存在相关免疫染色。早期纤连蛋白的表达高于层粘连蛋白（p<0.05）。随着妊娠进展，在腔上皮细胞和腺上皮细胞顶端观察到整合素β4和α5的时空免疫染色（p<0.05）。体外实验结果显示，人绒毛膜促性腺激素（hCG）刺激层粘连蛋白表达，下调整合素β4表达，而雌二醇降低了Ishikawa细胞中纤连蛋白的表达。hCG抑制培养的子宫内膜基质细胞中纤连蛋白的表达。我们的结果表明，纤连蛋白负责诱导蜕膜细胞分化，整合素不同的时空表达可能在着床过程中发挥作用。我们的体外实验结果表明，细胞外基质重塑和整合素转换的调节至少部分受生殖激素调控。
