快速检测唾液中乳酸脱氢酶和疟原虫恶性疟原虫基因型在儿童急性无并发症疟疾中的应用。
Rapid detection of lactate dehydrogenase and genotyping of Plasmodium falciparum in saliva of children with acute uncomplicated malaria.
机构信息
Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan, Ibadan, Nigeria.
出版信息
Am J Trop Med Hyg. 2010 Sep;83(3):496-501. doi: 10.4269/ajtmh.2010.10-0166.
The diagnosis of malaria in biological fluids other than blood using non-invasive, rapid diagnostic techniques provides a valuable approach in case management and epidemiological studies of malaria. Rapid detection of Plasmodium falciparum lactate dehydrogenase (pLDH) in saliva samples from 130 of 144 children with microscopically confirmed P. falciparum infection was evaluated using Optimal-IT dipsticks. Genotyping of parasites was also performed in saliva and blood samples from a cohort of patients by polymerase chain reaction (PCR). The sensitivity of the dipstick in whole-blood, whole-saliva, or supernatant of spun saliva samples was 97.2%, 77.9%, and 48.4%, respectively. The sensitivity of the dipstick in whole-saliva samples was significantly higher than in supernatant of spun saliva samples (P < 0.0005). Mutant T76 allele was detectable in 60% and 57% of blood and saliva samples, respectively. This finding shows rapid detection of pLDH in patient saliva.
在血液以外的生物体液中使用非侵入性、快速诊断技术来诊断疟疾，为疟疾的病例管理和流行病学研究提供了一种有价值的方法。使用 Optimal-IT 检测条评估了从 144 名经显微镜确认有恶性疟原虫感染的儿童中的 130 名儿童的唾液样本中疟原虫乳酸脱氢酶（pLDH）的快速检测。还通过聚合酶链反应（PCR）在唾液和血液样本中对寄生虫进行基因分型。检测条在全血、全唾液或离心唾液上清液中的灵敏度分别为 97.2%、77.9%和 48.4%。检测条在全唾液样本中的灵敏度明显高于离心唾液上清液（P < 0.0005）。突变 T76 等位基因在血液和唾液样本中的检出率分别为 60%和 57%。这一发现表明可以在患者唾液中快速检测到 pLDH。
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