Research Service, Veterans Affairs Medical Center, Minneapolis, Minnesota 55417, USA.
Antioxid Redox Signal. 2011 Jul 15;15(2):373-8. doi: 10.1089/ars.2010.3525. Epub 2011 Feb 3.
Endogenously produced hydrogen sulfide is thought to function as an intracellular messenger. There is, however, little information on tissue concentrations of free hydrogen sulfide, the putative messenger form of this molecule, versus that of the bound (acid-labile) form. The present report describes the application of a novel technique to measure free and acid-labile hydrogen sulfide in mouse tissues. Very low free hydrogen sulfide concentrations (<0.050 μmol/kg) were observed in brain, liver, blood, heart, kidney, striated muscle, and esophagus. Aortic concentrations of free hydrogen sulfide were 20 to 100 times greater than that of the other tissues. Acid-labile hydrogen sulfide concentrations were multiple orders of magnitude greater than that of the free form in every tissue other than aorta. Previous reports of tissue hydrogen sulfide concentrations of 30 to >100 μmol/kg measured bound rather than free hydrogen sulfide, the observation that aorta contains anomalously high free hydrogen sulfide concentrations lends support for a vasodilator function for this molecule, and the very low free hydrogen sulfide concentrations in most tissues seemingly requires intermediation of a yet to be described receptor-like mechanism if this molecule is to serve as a gasotransmitter.
内源性产生的硫化氢被认为是一种细胞内信使。然而，关于这种分子的游离（酸不稳定）形式与结合（酸不稳定）形式的组织浓度的信息却很少。本报告描述了一种测量小鼠组织中游离和酸不稳定硫化氢的新技术。在脑、肝、血、心、肾、横纹肌和食管中观察到极低的游离硫化氢浓度（<0.050 μmol/kg）。主动脉中的游离硫化氢浓度是其他组织的 20 到 100 倍。除了主动脉之外，在其他组织中，酸不稳定的硫化氢浓度比游离形式高出多个数量级。先前报道的组织硫化氢浓度为 30 至>100 μmol/kg，测量的是结合而不是游离的硫化氢，观察到主动脉中含有异常高的游离硫化氢浓度，这为这种分子的血管舒张功能提供了支持，而大多数组织中极低的游离硫化氢浓度，如果这种分子要作为一种气体递质，似乎需要一种尚未描述的受体样机制的中介作用。
