Kang Yijun, Cheng Jie, Mei Lijuan, Yin Shixue
College of Agronomy, Yangzhou University, Yangzhou 225009, China.
Wei Sheng Wu Xue Bao. 2010 Jul;50(7):853-61.
The interaction between plant growth promoting rhizobacteria (PGPR) and plants can be unstable, PGPR with PGP activities may be well adapted to particular soil environment. Based on this, we isolated and identified PGPRs from different rhizosphere soils according to their multiple mechanisms.
Preliminary screening of PGPRs under the premises of PGPR may having the abilities of N2-fixing, phosphate and potassium solubilization, and resistance against six common pathogenic fungi as well as rhizosphere colonization. After that, multiple PGP activities were detected in vitro. Finally, PGPRs were classified and identified by combining physiological and biochemical tests and 16S rRNA gene sequence analysis.
Fourteen strains having various mechanisms of PGP activities such as NH3, IAA, HCN, siderophore, antibiotics production, phosphate and potassium solubilization, and N2-fixing were isolated from different rhizosphere soils in Yangzhou and Yancheng, Jiangsu province. These 14 isolates could be identified as Pseudomonas (7 isolates), Paenibacillus (3 isolates), Bacillus (2 isolates), Burkholderia (1 isolate) and Erwinia (1 isolate).
Isolates with multiple PGP activities can also be rhizospheric competent, able to survive and colonize in the rhizosphere, providing promising isolates for PGPRs combination to resolve the challenges in field application of PGPR.
植物促生根际细菌（PGPR）与植物之间的相互作用可能不稳定，具有植物促生活性的PGPR可能很好地适应特定的土壤环境。基于此，我们根据其多种机制从不同的根际土壤中分离并鉴定了PGPR。
在PGPR可能具有固氮、解磷、解钾能力，对六种常见致病真菌具有抗性以及根际定殖能力的前提下，对PGPR进行初步筛选。之后，在体外检测多种植物促生活性。最后，结合生理生化试验和16S rRNA基因序列分析对PGPR进行分类和鉴定。
从江苏省扬州和盐城不同的根际土壤中分离出14株具有多种植物促生活性机制的菌株，如氨、吲哚乙酸、氰化氢、铁载体、抗生素产生、解磷、解钾和固氮。这14株分离物可鉴定为假单胞菌属（7株）、类芽孢杆菌属（3株）、芽孢杆菌属（2株）、伯克霍尔德菌属（1株）和欧文氏菌属（1株）。
具有多种植物促生活性的分离物也具有根际定殖能力，能够在根际存活和定殖，为PGPR组合解决PGPR田间应用中的挑战提供了有前景的分离物。
