Gabrion J B, Barrière H, Nguyen Than Dao B, Chambard M, Mauchamp J, Regnouf F, Pradel L A
Laboratoire de Neurobiologie Endocrinologique, UA CNRS 1197, Université de Montpellier II, France.
Eur J Cell Biol. 1990 Aug;52(2):282-90.
A fodrin-like protein purified from porcine thyroid cells and characterized by its properties identical to those of pig brain spectrin (F. Regnouf et al., Eur. J. Biochem. 153, 313-319 (1985)) has been localized by immunofluorescence and electron immunocytochemistry in porcine and rat thyroid. Fodrin-like polypeptides were detected in subplasmalemmal meshworks of microfilaments attached to isolated or in situ plasma membranes. In resting cells, fodrin was found under apical and basolateral membrane domains, whereas it was always absent under the pseudopod membrane domain induced by acute TSH stimulation in vitro, using monolayers of porcine cultured cells attached to collagen permeable substrates, as well as in vivo, using rats intravenously treated with TSH. Thyroid fodrin could be involved in exocytosis and membrane stabilization which occurs during the formation of pseudopods induced by TSH stimulation.
从猪甲状腺细胞中纯化出一种类血影蛋白，其特性与猪脑血影蛋白相同（F. 勒努夫等人，《欧洲生物化学杂志》153卷，313 - 319页（1985年）），已通过免疫荧光和免疫电子细胞化学方法在猪和大鼠甲状腺中定位。在附着于分离或原位质膜的微丝的亚质膜网络中检测到类血影蛋白多肽。在静息细胞中，血影蛋白存在于顶端和基底外侧膜结构域之下，而在体外使用附着于可渗透胶原底物的猪培养细胞单层，以及在体内使用经促甲状腺激素静脉注射处理的大鼠时，在急性促甲状腺激素刺激诱导的伪足膜结构域之下却始终不存在。甲状腺血影蛋白可能参与促甲状腺激素刺激诱导伪足形成过程中发生的胞吐作用和膜稳定作用。
