Rungger E, Stepanian E, Gilodi N
Laboratoire de Microscopie électronique, Clinique d'Ophthalmologie Universitaire, Genève.
Klin Monbl Augenheilkd. 1991 May;198(5):408-10. doi: 10.1055/s-2008-1045994.
Fodrin, a member of the spectrin family, is abundantly found in brain and lens. Based on its often observed peripheral cellular distribution, it is generally believed that fodrin - like other spectrins - participates in the linkage of membrane components to cytoskeletal elements providing their anchorage to distinct, highly specialized membrane regions. To investigate the role of fodrin in the vertebrate (amphibian) retina, we have produced polyclonal antibodies in rabbits against fodrin purified from veal lens. The distribution of fodrin within the retina of Xenopus and Rana has been assessed by indirect immunofluorescence on cryostat sections and on isolated epithelial cells. In general, the staining pattern reflects the structural and functional polarity of the retinal layers. Fodrin is found in axons, dendrites and synapses of neurons, in the inner segment of photoreceptors, and as a peripheral ring of the epithelial cells. This study represents the first step of an investigation on the development and differentiation of specific, fodrin-containing membrane regions in the amphibian retina.
血影蛋白是血影蛋白家族的一员，在大脑和晶状体中大量存在。基于其常见的外周细胞分布，人们普遍认为血影蛋白——与其他血影蛋白一样——参与膜成分与细胞骨架元件的连接，使它们锚定在不同的、高度特化的膜区域。为了研究血影蛋白在脊椎动物（两栖动物）视网膜中的作用，我们用从牛肉晶状体中纯化的血影蛋白在兔子体内制备了多克隆抗体。通过对冷冻切片和分离的上皮细胞进行间接免疫荧光检测，评估了非洲爪蟾和林蛙视网膜内血影蛋白的分布。一般来说，染色模式反映了视网膜各层的结构和功能极性。血影蛋白存在于神经元的轴突、树突和突触中，存在于光感受器的内段，以及上皮细胞的外周环中。本研究是对两栖动物视网膜中特定的、含血影蛋白的膜区域的发育和分化进行研究的第一步。
