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洋葱鳞茎表皮细胞中移动和固定的内质网:共聚焦激光扫描显微镜的短期和长期观察

Mobile and immobile endoplasmic reticulum in onion bulb epidermis cells: short- and long-term observations with a confocal laser scanning microscope.

作者信息

Knebel W, Quader H, Schnepf E

机构信息

Zellenlehre, Fakultät für Biologie, Universität Heidelberg, Bundesrepublik Deutschland.

出版信息

Eur J Cell Biol. 1990 Aug;52(2):328-40.

PMID:2081534
Abstract

The endoplasmic reticulum (ER) of onion bulb scale epidermis cells consists of long, tubular strands lying deep in the cytoplasm which move quickly and a less mobile peripheral network of tubules and cisternae that change in position, shape and size but that also have immobile, fixed, sites (IFSs). IFSs occur in junctions, at vertexes and at blind endings of tubules as well as at the edges and the surface of cisternae. They are regularly arranged in helicoidal rows and may be knot- or ring-like in structure. They become enlarged by treatment with oryzalin but not with colchicine. They persist for long times (for more than 30 min); together with pulling forces, the surface tension and other factors, they determine the configuration and motion of the peripheral network. New polygons of the network are mainly formed by the development of new tubules that become joined with other parts of the network. Polygons disappear by contraction and fusion of tubules. The inner, rapidly moving ER tubules remain connected with the peripheral network over longer distances by sliding junctions. Cytochalasin D causes an accumulation of the ER into patches, a fusion of tubules into cisternae and changes in shape, which indicate the loss of pulling forces. In contrast to animal cells (but like the movement of the inner tubular strands), the latter is dependent upon the actomyosin system; microtubules are not involved. Despite the differences in the organizing components, the peripheral ER in onion bulb scale epidermis cells and that of the borders of cultured animal cells are similar in morphology and motility.

摘要

洋葱鳞茎表皮细胞的内质网(ER)由位于细胞质深处的长管状丝组成,这些丝移动迅速,还有一个移动性较差的周边网络,由小管和潴泡组成,其位置、形状和大小会发生变化,但也有固定不动的位点(IFS)。IFS出现在小管的连接处、顶点和盲端,以及潴泡的边缘和表面。它们以螺旋状排列,结构可能呈结状或环状。用oryzalin处理后它们会增大,但用秋水仙碱处理则不会。它们能长时间持续存在(超过30分钟);与拉力、表面张力和其他因素一起,它们决定了周边网络的形态和运动。网络的新多边形主要由新形成的小管发展而来,这些小管会与网络的其他部分相连。多边形通过小管的收缩和融合而消失。内部快速移动的内质网小管通过滑动连接与周边网络在较长距离上保持连接。细胞松弛素D会导致内质网聚集形成斑块,小管融合形成潴泡并发生形状变化,这表明拉力丧失。与动物细胞不同(但与内部管状丝的运动类似),后者依赖于肌动球蛋白系统;微管不参与其中。尽管在组织成分上存在差异,但洋葱鳞茎表皮细胞中的周边内质网与培养动物细胞边界处的周边内质网在形态和运动性上相似。

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