Foissner Ilse
Department of Cell Biology, Division of Plant Physiology, University of Salzburg, Hellbrunnerstrasse 34, 5020 Salzburg, Austria.
Protoplasma. 2009 Dec;238(1-4):47-58. doi: 10.1007/s00709-009-0072-5. Epub 2009 Oct 1.
The staining pattern of 1,2-bis(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-undecanoyl)-sn-glycero-3-phosphocholine (Bodipy PC) was investigated in internodal cells of the green alga Chara corallina. Ten minutes after dye addition, Bodipy-PC-derived fluorescence appeared in lipid droplets and after 1 h in the cortical endoplasmic reticulum (ER) and in the inner ER tubes. Staining of the ER required energy but was independent of an intact actin or microtubule cytoskeleton and independent of vesicular endocytosis. The size of the lipid droplets varied between 0.25 microm in elongating cells and 3.2 microm in senescent internodes. They moved together with or along the cortical ER cisternae in a cytoskeleton-independent manner or remained immobile up to several minutes. Detachment of lipid droplets from the cortical ER or fusion of lipid droplets was never observed. The results of this study suggest that Bodipy PC is a valuable, less toxic alternative to 3,3'-dihexyloxacarbocyanine iodide (DiOC6) staining of the ER in Chara. They confirm an earlier report about microtubule-dependent cortical ER morphology and dynamics in elongating internodes and offer new perspectives for the study of organelle interactions.
研究了1,2-双(4,4-二氟-5,7-二甲基-4-硼-3a,4a-二氮杂-s-茚并-3-十一烷酰基)-sn-甘油-3-磷酸胆碱（Bodipy PC）在绿藻轮藻节间细胞中的染色模式。添加染料10分钟后，Bodipy-PC产生的荧光出现在脂滴中，1小时后出现在皮质内质网（ER）和内质网管中。内质网的染色需要能量，但与完整的肌动蛋白或微管细胞骨架无关，也与囊泡内吞作用无关。脂滴的大小在伸长细胞中为0.25微米，在衰老节间中为3.2微米。它们以与细胞骨架无关的方式与皮质内质网池一起移动或沿着皮质内质网池移动，或者在几分钟内保持不动。从未观察到脂滴与皮质内质网分离或脂滴融合。本研究结果表明，Bodipy PC是轮藻内质网3,3'-二己基氧杂羰花青碘化物（DiOC6）染色的一种有价值、毒性较小的替代方法。它们证实了早期关于伸长节间中微管依赖性皮质内质网形态和动力学的报道，并为细胞器相互作用的研究提供了新的视角。
