National Parkinson Foundation Centre of Excellence Research Laboratory, Clinic and Policlinic for Psychiatry, Psychosomatic and Psychotherapy, University of Würzburg, Würzburg, Germany.
Neurochem Int. 2010 Dec;57(7):819-22. doi: 10.1016/j.neuint.2010.08.020. Epub 2010 Sep 15.
Brain bank centers around the world attempt to standardize postmortem brain collection and quality control. Antemortem as well postmortem factors may influence tissue quality. Previously, we could demonstrate that increased tryptophan (TRP) levels significantly correlate to prolonged postmortem interval (PMI) and storage duration, whereas pH-value altered merely as consequence of prolonged agonal state and ischemic brain damage additionally to repeated freeze and thaw cycles. Therefore, we aimed to investigate in artificial PMI conditions, with three brain tissue storage temperatures (4°C, room temperature and 37°C) as well as oxidizing conditions (open/close tube), whether TRP levels and pH-value alter. We could confirm that prolonged PMI at higher storage temperatures and oxidizing conditions significantly correlate to increased TRP levels, while pH-value did not correlate at all. In conclusion, from these results PMI intervals until autopsy should be kept as short as possible and storage until autopsy should be at 4°C in order to preserve brain tissue quality as much as possible.
世界各地的脑库中心试图使死后大脑采集和质量控制标准化。生前和死后的因素都可能影响组织质量。此前，我们已经证明，色氨酸（TRP）水平的升高与死后间隔时间（PMI）和储存时间的延长显著相关，而 pH 值的改变仅仅是由于延长的濒死状态和缺血性脑损伤以及反复的冷冻和解冻循环的结果。因此，我们旨在研究在人工 PMI 条件下，三种脑组织储存温度（4°C、室温及 37°C）以及氧化条件（管开放/关闭）下，TRP 水平和 pH 值是否会发生改变。我们可以确认，在较高储存温度和氧化条件下，PMI 时间延长与 TRP 水平升高显著相关，而 pH 值则完全没有相关性。总之，从这些结果来看，尸检前的 PMI 间隔时间应尽可能缩短，并且在尸检前应将储存温度保持在 4°C，以尽可能地保存脑组织质量。
