Madeira M D, Pereira A, Cadete-Leite A, Paula-Barbosa M M
Department of Anatomy, Oporto Medical School, Porto, Portugal.
J Anat. 1990 Aug;171:41-56.
In previous quantitative studies we demonstrated that the volumes of the cerebellar and hippocampal granular layers, as well as their total number of cells, were reduced in 30 days old hypothyroid rats. We decided to extend these studies to the prelimbic subarea of the medial prefrontal cortex using the same morphometric procedures. The cortical volume and the total number of neurons of its Layer III were determined. After correcting for the tissue shrinkage factor, it was found that the volume of the entire prelimbic cortex and that of its Layer III was smaller in hypothyroid rats than in controls. Conversely, the number of neurons per unit volume of cortical Layer III was greater in hypothyroids. This indicates that there is a markedly increased cell packing density, probably related to the neuropil impoverishment, as was described by other authors almost forty years ago for the sensorimotor and visual cortices, applying inaccurate quantitative methods.
在之前的定量研究中，我们证明了30日龄甲状腺功能减退大鼠的小脑和海马颗粒层体积以及细胞总数均减少。我们决定使用相同的形态测量方法将这些研究扩展到内侧前额叶皮质的前边缘亚区。测定了皮质体积及其第III层的神经元总数。校正组织收缩因子后发现，甲状腺功能减退大鼠的整个前边缘皮质及其第III层的体积均小于对照组。相反，甲状腺功能减退大鼠皮质第III层单位体积内的神经元数量更多。这表明细胞堆积密度显著增加，可能与神经毡减少有关，就像近四十年前其他作者使用不准确的定量方法描述感觉运动和视觉皮质时那样。
