对104只患有自然发生的内源性葡萄膜炎的猫的血液和房水样本进行巴尔通体属、猫疱疹病毒-1和刚地弓形虫PCR检测的结果。
Bartonella species, feline herpesvirus-1, and Toxoplasma gondii PCR assay results from blood and aqueous humor samples from 104 cats with naturally occurring endogenous uveitis.
作者信息
Powell Cynthia C, McInnis Carey L, Fontenelle Jennifer P, Lappin Michael R
机构信息
Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, USA.
出版信息
J Feline Med Surg. 2010 Dec;12(12):923-8. doi: 10.1016/j.jfms.2010.07.003.
Toxoplasma gondii, Bartonella henselae and feline herpesvirus-1 (FHV-1) have been implicated as causative agents in feline uveitis. The usefulness of serum and aqueous humor (AH) antibody testing for these agents is limited as antibodies can be detected in both healthy cats and cats with uveitis. Very few studies using polymerase chain reaction (PCR) assays to amplify organism DNA from samples from cats with uveitis have been performed. In this study, assays to detect T gondii antibodies, feline leukemia virus antigen, feline immunodeficiency virus antibody, and Bartonella species antibodies were performed on serum and PCR assays for amplification of T gondii, Bartonella species, and FHV-1 DNA were performed on blood and AH samples from 104 cats with endogenous uveitis and 19 healthy cats. Results suggest the addition of the PCR assay to the diagnostic work-up for cats with uveitis will increase the detection of T gondii and FHV-1; however, the diagnostic usefulness of these additional data is not clear.
刚地弓形虫、汉赛巴尔通体和猫疱疹病毒-1（FHV-1）被认为是猫葡萄膜炎的病原体。血清和房水（AH）抗体检测对这些病原体的作用有限，因为在健康猫和患有葡萄膜炎的猫中均可检测到抗体。很少有研究使用聚合酶链反应（PCR）检测法从患有葡萄膜炎的猫的样本中扩增病原体DNA。在本研究中，对104只患有内源性葡萄膜炎的猫和19只健康猫的血清进行了检测刚地弓形虫抗体、猫白血病病毒抗原、猫免疫缺陷病毒抗体和巴尔通体属抗体的检测，并对血液和房水样本进行了PCR检测以扩增刚地弓形虫、巴尔通体属和FHV-1 DNA。结果表明，在对患有葡萄膜炎的猫的诊断检查中增加PCR检测将提高刚地弓形虫和FHV-1的检测率；然而，这些额外数据的诊断价值尚不清楚。
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