Caruncho H J, Rodriguez-Moldes I, Anadón R
Departamento de Biologia Fundamental, Universidad de Santiago de Compostela, Spain.
J Hirnforsch. 1990;31(5):575-84.
Monoaminergic neurons in the hypothalamic nucleus recessi posterioris of the rainbow trout were studied by freeze-fracturing. The size and distribution of intramembrane particles in the nuclear and plasma membranes and the density of nuclear pores in the nuclear envelope were determined. The density of nuclear pores in these cells was significantly greater than in the non-granulated neurons of this nucleus. The density of IMPs in the P face of the inner nuclear membrane was significantly greater than in the P face of the outer nuclear membrane; however in the E faces the density is greater in the outer nuclear membrane but the differences are not statistically significant. In both membranes, the proportion of large particles (greater than 9.6 nm) was greater in the E than in the P faces. The coefficient of partition between the plasma membrane faces was 1.97 +/- 0.24 while in non-granulated neurons was 4.45 +/- 0.51. There are significant differences among these values. Interestingly, the coefficient of partition of the plasma membrane in monoaminergic neurons was lesser than in some other types of neurons. Postsynaptic areas or other membrane specializations were not found on the cell body plasma membrane, which corroborates the results of conventional transmission electron microscopy.
通过冷冻蚀刻技术研究了虹鳟下丘脑后隐窝核中的单胺能神经元。测定了核膜和质膜中膜内颗粒的大小和分布以及核被膜中核孔的密度。这些细胞中核孔的密度显著高于该核中无颗粒神经元的核孔密度。内核膜P面的整合膜蛋白（IMPs）密度显著高于外核膜P面；然而，在外核膜的E面，密度更高，但差异无统计学意义。在这两种膜中，E面大于9.6nm的大颗粒比例高于P面。质膜面之间的分配系数为1.97±0.24，而在无颗粒神经元中为4.45±0.51。这些值之间存在显著差异。有趣的是，单胺能神经元中质膜的分配系数低于其他一些类型的神经元。在细胞体质膜上未发现突触后区域或其他膜特化结构，这与传统透射电子显微镜的结果一致。
