Karasawa Y, Okisaka S
Department of Ophthalmology, National Defense Medical College, Saitama, Japan.
Jpn J Ophthalmol. 1990;34(4):479-93.
Conditioned medium wherein bovine retinal pigment epithelial cells have been cultured (RPE-CM) inhibited proliferation of the capillary endothelial cells (CEC) of the bovine adrenal gland. The RPE-CM was fractionated into three fractions; molecular weight of more than 30 kilo Daltons (kDa) (30 kDa fraction), between 10 and 30 kDa (10 kDa-30 kDa fraction), and less than 10 kDa (10 kDa fraction). Each fraction was tested for its effect on the proliferation, morphology and movement of the CEC. The proliferation of CEC was inhibited in the more than 30 kDa fraction and less than 10 kDa fraction, but not in the 10 kDa-30 kDa fraction. The RPE-CM changed morphology of the CEC into slender shape. This morphological change was observed only in the more than 30 kDa fraction, and the CEC in the other fractions maintained normal morphology. When the CEC proliferation was arrested by hydroxyurea, RPE-CM and the more than 30 kDa fraction did not change morphology. Unfractionated RPE-CM and the more than 30 kDa fraction which changed the morphology of the CEC also inhibited movement of the CEC, such as the migration of cells from a confluent cell layer and single cell movement. These findings suggested that the RPE in culture secrete soluble anti-angiogenic factors into the medium.
已培养过牛视网膜色素上皮细胞的条件培养基（RPE-CM）抑制了牛肾上腺毛细血管内皮细胞（CEC）的增殖。RPE-CM被分离成三个组分；分子量大于30千道尔顿（kDa）（30 kDa组分）、10至30 kDa之间（10 kDa - 30 kDa组分）以及小于10 kDa（10 kDa组分）。测试了每个组分对CEC增殖、形态和迁移的影响。CEC的增殖在大于30 kDa组分和小于10 kDa组分中受到抑制，但在10 kDa - 30 kDa组分中未受抑制。RPE-CM将CEC的形态改变为细长形。这种形态变化仅在大于30 kDa组分中观察到，其他组分中的CEC保持正常形态。当CEC增殖被羟基脲阻滞时，RPE-CM和大于30 kDa组分未改变形态。未分级的RPE-CM和改变CEC形态的大于30 kDa组分也抑制了CEC的迁移，如细胞从汇合细胞层的迁移和单细胞移动。这些发现表明培养中的RPE向培养基中分泌可溶性抗血管生成因子。
