Dipartimento Farmaco-biologico, Università degli Studi di Bari, Bari, Italia.
J Bioenerg Biomembr. 2010 Oct;42(5):371-9. doi: 10.1007/s10863-010-9308-5.
Here, the oxoglutarate carrier, already isolated from various sources and described in the literature, has been purified from rat brain and reconstituted in proteoliposomes for an accurate kinetic study. The rate of uptake of labelled oxoglutarate and malate has been measured in various conditions, essentially in double substrate experiments. The data so obtained fit the hypothesis that the carrier operates by a uniport-exchange mechanism and provide significant values for the kinetic constants and the equilibrium constants implied in the process. Their analysis leads to the conclusion that the carrier is maximally efficient in the exchange between external malate and internal oxoglutarate, as required by the malate/aspartate shuttle, which should be the main role of the oxoglutarate carrier in brain mitochondria.
这里，已从各种来源分离并在文献中描述的草酰乙酸载体已从鼠脑中纯化，并在类脂体中重建以进行准确的动力学研究。已经在各种条件下测量了标记的草酰乙酸和苹果酸的摄取速率，主要是在双底物实验中。所得数据符合载体通过单载体交换机制起作用的假设，并为该过程中涉及的动力学常数和平衡常数提供了有意义的值。它们的分析得出的结论是，载体在外部苹果酸和内部草酰乙酸之间的交换中效率最高，这是苹果酸天冬氨酸穿梭所必需的，这应该是脑线粒体中草酰乙酸载体的主要作用。
