Ye Shuangfeng, Wang Mo, Liu Liangqing, Zhang Huizhi
Institute of Medicinal Plant of Huazhong Agricultural University, Wuhan 430070, China.
Zhongguo Zhong Yao Za Zhi. 2010 Jun;35(11):1382-5.
To establish the seed quality grading standard of artificial tuber of Pinellia ternata.
Hundred-grain weight, moisture content, germination rate, viability, single-grain diameter, purity and visual form of mature artificial tuber seed from tissue culture of P. ternate were determined. The key indicator and reference indicators for artificial tubers quality grading were defined statistically by SPSS analysis.
Germination rate and viability were the primary indicators, hundred-grain weight, moisture content and purity were important reference indicators.
The quality of each grade should reach the following requirements: for first grade, hundred-grain weight > 10 g, moisture content > 70%-80% , germination rate > 80%, viability > 85%, single-grain diameter > 0.7 cm, purity > 80%; for second grade, hundred-grain weight 8-10 g, moisture content > 70%-80%, germination rate 60%-80%, viability 70%-85%, single-grain diameter 0.3-0.7 cm, purity 75%-80%; moisture content > 70%-80%, and the others don't reach second grade was the third grade.
建立半夏人工块茎的种子质量分级标准。
测定半夏组织培养成熟人工块茎种子的百粒重、含水量、发芽率、生活力、单粒直径、纯度及外观形态。通过SPSS分析,统计确定人工块茎质量分级的关键指标和参考指标。
发芽率和生活力为主要指标,百粒重、含水量和纯度为重要参考指标。
各等级质量应达到以下要求:一级,百粒重>10 g,含水量>70%-80%,发芽率>80%,生活力>85%,单粒直径>0.7 cm,纯度>80%;二级,百粒重8-10 g,含水量>70%-80%,发芽率60%-80%,生活力70%-85%,单粒直径0.3-0.7 cm,纯度75%-80%;含水量>70%-80%,其他未达二级标准的为三级。