[明胶法检测两种注射液中细菌内毒素的含量]
[Content detection of bacterial endotoxin in two kinds of injection by gelatin technique].
作者信息
Gao Shuangrong, Liu Ting, Guo Jing, Yi Yan, Hao Ran, Li Chunying, Cao Chunyu, Zhao Yong, Hui Lianqiang, Liang Aihua
机构信息
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2010 Jun;35(11):1405-9.
OBJECTIVE
To detect content of bacterial endotoxin in Yuxingcao and Qingkailing injections by specific and nonspecific tachypleus amebocyte lysate technique for in order to investigate the feasibility of specific tachypleus amebocyte lysate technique for detecting bacterial endotoxin in traditional Chinese drug injections.
METHOD
Different batches of Yuxingcao and Qingkailing injections were detected by specific and nonspecific tachypleus amebocyte lysate kits.
RESULT
Yuxingcao injection could be detected by specific and nonspecific tachypleus amebocyte lysate technique, Whereas Qingkailing injection could be detected only by specific tachypleus amebocyte lysate.
CONCLUSION
Using specific tachypleus amebocyte lysate as a substitute for nonspecific tachypleus amebocyte lysate is an effective method for detecting content of bacterial endotoxin in Qingkailing injection.
目的
采用特异性和非特异性鲎试剂法检测鱼腥草注射液和清开灵注射液中细菌内毒素的含量，以探讨特异性鲎试剂法检测中药注射液中细菌内毒素的可行性。
方法
应用特异性和非特异性鲎试剂试剂盒对不同批次的鱼腥草注射液和清开灵注射液进行检测。
结果
鱼腥草注射液采用特异性和非特异性鲎试剂法均能检测出细菌内毒素，而清开灵注射液仅采用特异性鲎试剂法能检测出细菌内毒素。
结论
用特异性鲎试剂替代非特异性鲎试剂检测清开灵注射液中细菌内毒素的含量是一种有效方法。
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