Usawattanakul W, Tharavanij S, Limsuwan A
Southeast Asian J Trop Med Public Health. 1979 Mar;10(1):13-7.
Amoebocyte lysate from the horseshoe crabs (Tachypleus gigas) which abounds in the Gulf of Thailand was used to detect endotoxin in patients with Gram-negative bacteremia, in patients with Gram-positive bacterial infections as well as in the control. The Tachypleus lysate test (TLT) was positive in 94.4% of 36 patients with Gram-negative bacteremia before initiation of antibiotic therapy. Only 4% of 50 healthy individuals were positive and all 7 patients with Gram-positive bacterial infections were negative. The threshold sensitivity of TLT was 0.625 micrograms endotoxin per ml of the plasma. In comparison with the commercial Limulus lysate test (LLT), the TLT was slightly more sensitive in exhibiting higher grade of reaction, eventhough the threshold sensitivity was the same.
来自泰国湾盛产的鲎（巨鲎）的变形细胞溶解物被用于检测革兰氏阴性菌血症患者、革兰氏阳性菌感染患者以及对照组中的内毒素。36例革兰氏阴性菌血症患者在开始抗生素治疗前，鲎溶解物试验（TLT）的阳性率为94.4%。50名健康个体中只有4%呈阳性，7例革兰氏阳性菌感染患者均为阴性。TLT的阈值敏感性为每毫升血浆0.625微克内毒素。与市售的鲎试剂试验（LLT）相比，尽管阈值敏感性相同，但TLT在显示更高等级反应方面略更敏感。
