Institute of Environmental Science and Research Limited (ESR), Kenepuru Science Centre, Porirua, New Zealand.
Epidemiol Infect. 2011 Jul;139(7):1050-9. doi: 10.1017/S0950268810002025. Epub 2010 Sep 8.
The discriminatory power of multiple-locus variable-number tandem-repeat analysis (MLVA) needs to be evaluated for all Salmonella enterica subspecies enterica serovar Typhimurium (S. Typhimurium) phage types so that the power of this methodology is understood and results can be interpreted correctly during outbreak investigations. We evaluated the ability of MLVA to characterize four definitive phage types (DT) problematic in New Zealand. MLVA discriminated between DT104 isolates although there was very limited variation in the MLVA profiles for isolates with an RDNC phage type (reacts but does not conform to a recognized Typhimurium phage pattern) first observed in New Zealand's Enteric Reference Laboratory in May 2006. Most DT101 isolates had indistinguishable MLVA profiles or profiles that differed at one or two loci. This was also observed in DT160 isolates. MLVA may not identify all common-source outbreaks although it provided valuable data when applied to case isolates from two S. Typhimurium outbreaks.
多位点可变数目串联重复分析（MLVA）的区分能力需要针对所有肠沙门氏菌亚种肠炎血清型鼠伤寒（S. Typhimurium）噬菌体型进行评估，以便了解该方法的效力，并在暴发调查期间正确解释结果。我们评估了 MLVA 对新西兰存在的四个确定噬菌体型（DT）进行特征描述的能力。MLVA 可区分 DT104 分离株，尽管 2006 年 5 月在新西兰肠道参考实验室首次观察到的具有 RDNC 噬菌体型（反应但不符合公认的鼠伤寒噬菌体模式）的分离株的 MLVA 图谱变化非常有限。大多数 DT101 分离株具有不可区分的 MLVA 图谱或仅在一个或两个位点存在差异的图谱。DT160 分离株也观察到这种情况。MLVA 可能无法识别所有同源暴发，尽管在应用于两个 S. Typhimurium 暴发的病例分离株时提供了有价值的数据。
