Torpdahl Mia, Sørensen Gitte, Lindstedt Bjørn-Arne, Nielsen Eva Møller
Department of Bacteriology, Mycology and Parasitology, Statens Serum Institut, Copenhagen, Denmark.
Emerg Infect Dis. 2007 Mar;13(3):388-95. doi: 10.3201/eid1303.060460.
In Denmark, as part of the national laboratory-based surveillance system of human enteric infections, all Salmonella enterica serotype Typhimurium isolates are currently subtyped by using phage typing, antimicrobial resistance profiles, and pulsed-field gel electrophoresis (PFGE). We evaluated the value of real-time typing that uses multiple-locus variable-number tandem-repeats analysis (MLVA) of S. Typhimurium to detect possible outbreaks. Because only a few subtypes identified by PFGE and phage typing account for most infections, we included MLVA typing in the routine surveillance in a 2-year period beginning December 2003. The 1,019 typed isolates were separated into 148 PFGE types and 373 MLVA types. Several possible outbreaks were detected and confirmed. MLVA was particularly valuable for discriminating within the most common phage types. MLVA was superior to PFGE for both surveillance and outbreak investigations of S. Typhimurium.
在丹麦，作为国家基于实验室的人类肠道感染监测系统的一部分，目前所有肠炎沙门氏菌鼠伤寒血清型分离株都通过噬菌体分型、抗菌药物耐药谱和脉冲场凝胶电泳（PFGE）进行亚型分析。我们评估了使用鼠伤寒沙门氏菌多位点可变数目串联重复序列分析（MLVA）进行实时分型以检测可能暴发的价值。由于PFGE和噬菌体分型鉴定出的少数亚型占大多数感染病例，我们在2003年12月开始的两年期间将MLVA分型纳入常规监测。1019株分型分离株被分为148种PFGE型和373种MLVA型。检测并确认了几起可能的暴发。MLVA在区分最常见的噬菌体类型方面特别有价值。在鼠伤寒沙门氏菌的监测和暴发调查中，MLVA均优于PFGE。
