Eisert W G, Müller T H
Department of Pharmacology, Dr K. Thomae GmbH, Biberach, FRG.
Thromb Res Suppl. 1990;12:65-72. doi: 10.1016/0049-3848(90)90441-e.
The effect of dipyridamole on the local antithrombotic activities of endothelium has been evaluated. Human whole blood was allowed to flow over an endothelial cell-derived extracellular matrix partially covered by human endothelial cells. Half-maximal suppression of platelet aggregate formation occurred with approximately 5 microM dipyridamole. Similarly, a pronounced inhibition of thrombus formation was observed by in vivo microscopy and computer-assisted morphometric analysis, after oral treatment of non-anesthetized hamsters with dipyridamole, 5 mg/kg. This strong suppression of thrombus formation was maintained in animals on a long-term cholesterol-supplemented diet. The antithrombotic potential of dipyridamole has been clearly demonstrated, both in vitro and in vivo using these more complex approaches employing quantitative microscopy.
已评估双嘧达莫对内皮局部抗血栓形成活性的影响。使人体全血在部分被人内皮细胞覆盖的内皮细胞衍生细胞外基质上流动。血小板聚集形成的半数最大抑制浓度约为5微摩尔双嘧达莫。同样，在用5毫克/千克双嘧达莫口服处理未麻醉的仓鼠后，通过体内显微镜检查和计算机辅助形态计量分析观察到血栓形成受到明显抑制。在长期补充胆固醇饮食的动物中，这种对血栓形成的强烈抑制作用得以维持。使用这些采用定量显微镜检查的更复杂方法，已在体外和体内清楚地证明了双嘧达莫的抗血栓形成潜力。
