UPMC Univ Paris 06, UMR 7195 PECSA, Physicochimie des Electrolytes, Colloïdes, Sciences Analytiques, F-75005 Paris France.
Langmuir. 2010 Oct 5;26(19):15453-63. doi: 10.1021/la102532h.
The present study deals with the morphological modifications of giant dioleoyl phosphatidylcholine vesicles (DOPC GUVs) induced by the nonionic surfactant n-octyl β,D-glucopyranoside at sublytic levels, i.e., in the first steps of the vesicle-to-micelle transition process, when surfactant inserts into the vesicle bilayer without disruption. Experimental conditions were perfected to exactly control the surfactant bilayer composition of the vesicles, in line with former work focused on the mechanical properties of the membrane of magnetic-fluid-loaded DOPC GUVs submitted to a magnetic field. The purpose here was to systematically examine, in the absence of any external mechanical constraint, the dynamics of giant vesicle shape and membrane deformations as a function of surfactant partitioning between the aqueous phase and the lipid membrane, beforehand established by turbidity measurements from small unilamellar vesicles.
本研究探讨了在亚毒性水平下，非离子表面活性剂辛基-β-D-吡喃葡萄糖苷对巨型二油酰基磷脂酰胆碱囊泡（DOPC GUV）形态的影响。当表面活性剂插入囊泡双层而不破坏双层时，就会发生这种情况，这一过程处于囊泡向胶束转变的初始步骤。实验条件经过优化，以精确控制囊泡的表面活性剂双层组成，这与之前专注于磁场下负载磁流体的 DOPC GUV 膜机械性能的工作一致。这里的目的是在没有任何外部机械约束的情况下，系统地研究在预先通过小单层囊泡浊度测量确定的水相和脂质膜之间的表面活性剂分配的情况下，巨型囊泡形状和膜变形的动力学。
