Department of Addiction Rehabilitation and Social Welfare, Eulji University, Gyeonggi-do 461-713, Republic of Korea.
Psychiatry Res. 2011 Apr 30;186(2-3):427-30. doi: 10.1016/j.psychres.2010.07.028. Epub 2010 Sep 9.
Diurnal changes of brain-derived neurotrophic factor (BDNF) mRNA and protein contents have been demonstrated in the rat central nervous system. In humans, however, insufficient data are available on diurnal variation of BDNF. We investigated plasma and serum BDNF levels at three different times of the day (9 a.m., 1 p.m. and 5 p.m.) in 18 healthy men and 18 healthy women. BDNF levels were assessed using an enzyme-linked immunosorbent assay (ELISA). We detected significant diurnal variation in plasma BDNF level in men, with a constant decrease throughout the day. However, we could not find diurnal variation in either the plasma BDNF of women or the serum BDNF in both men and women. When performing analysis of covariance with the age and body mass index as covariates, we found no significant differences of plasma and serum BDNF levels of the two sexes at the three time points. In psychiatric studies of blood BDNF level, diurnal variation in plasma BDNF level should be considered with respect to gender differences.
脑源性神经营养因子 (BDNF) 的 mRNA 和蛋白质含量在大鼠中枢神经系统中呈现昼夜变化。然而，关于 BDNF 的昼夜变化，人类的相关数据还不够充分。我们在一天中的三个不同时间点（上午 9 点、下午 1 点和 5 点）对 18 名健康男性和 18 名健康女性的血浆和血清 BDNF 水平进行了研究。采用酶联免疫吸附测定法 (ELISA) 评估 BDNF 水平。我们发现男性的血浆 BDNF 水平存在明显的昼夜变化，全天持续下降。然而，我们并未发现女性的血浆 BDNF 或男性和女性的血清 BDNF 存在昼夜变化。当我们以年龄和体重指数为协变量进行协方差分析时，在三个时间点，我们未发现男女两性的血浆和血清 BDNF 水平存在显著差异。在涉及血液 BDNF 水平的精神科研究中，应该考虑到性别差异对血浆 BDNF 水平昼夜变化的影响。
