Kemal W W, Yamada T, Shike S
Department of Pediatrics, Kanazawa Medical University, Ishikawa-ken, Japan.
Acta Paediatr Jpn. 1990 Dec;32(6):633-44. doi: 10.1111/j.1442-200x.1990.tb00897.x.
To evaluate hypodense eosinophils known very little in children, peripheral blood from asthmatic, allergic-non-asthmatic and non-allergic children was evaluated by density Percoll gradient techniques. A significantly higher number of eosinophils were hypodense in asthmatic children (n = 24) compared with allergic non-asthmatic (n = 10) and non-allergic children (n = 13), namely 484 +/- 348 cells/40 microliters versus 113 +/- 109 and 39 +/- 61 (p less than 0.001) respectively. We also observed by light and electron microscopy that the hypodense eosinophils of asthmatic children were swollen and their granules were dispersed, but the normodense eosinophils of the same patient were small and compact. In three cases of severe asthmatic attack, hypodense eosinophils found on admission decreased in number after intravenous aminophylline therapy and relief of symptoms. Moreover, a decreased number of hypodense eosinophils were found in seven cases of allergic children (p less than 0.05) after 2 weeks of anti-allergic drug treatment associated with relieved symptoms. From these data we concluded that the presence of hypodense eosinophils in the peripheral blood might be related to the development of allergic symptoms and might participate in the pathophysiology of allergic diseases in children.
为评估儿童中了解甚少的低密度嗜酸性粒细胞，采用Percoll密度梯度技术对哮喘儿童、过敏性非哮喘儿童及非过敏性儿童的外周血进行了评估。与过敏性非哮喘儿童（n = 10）和非过敏性儿童（n = 13）相比，哮喘儿童（n = 24）的低密度嗜酸性粒细胞数量显著更多，分别为484±348个细胞/40微升，而过敏性非哮喘儿童和非过敏性儿童分别为113±109个细胞/40微升和39±61个细胞/40微升（p<0.001）。我们还通过光学显微镜和电子显微镜观察到，哮喘儿童的低密度嗜酸性粒细胞肿胀，其颗粒分散，但同一患者的正常密度嗜酸性粒细胞则小而致密。在3例严重哮喘发作患者中，入院时发现的低密度嗜酸性粒细胞数量在静脉注射氨茶碱治疗及症状缓解后减少。此外，7例过敏性儿童在接受2周抗过敏药物治疗且症状缓解后，低密度嗜酸性粒细胞数量减少（p<0.05）。根据这些数据我们得出结论，外周血中低密度嗜酸性粒细胞的存在可能与过敏症状的发生有关，并可能参与儿童过敏性疾病的病理生理过程。
