Microbiology Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil.
J Med Virol. 2010 Oct;82(10):1797-802. doi: 10.1002/jmv.21816.
A total of 162 rotavirus strains detected between 1996 and 2006 among individuals with diarrhea in Rio de Janeiro, Brazil, were analyzed by multiple-gene genotyping. Characterization of strains was done by RT-PCR assay for amplification and typing of the VP7-, VP4-, VP6-, and NSP4-encoding genes. Overall, 139 (85.8%) strains belonged to the common group A rotavirus combinations: 67 (41.4%) belonged to genotype G1-P[8]-I1-E1; 18 (11.1%) were G2-P[4]-I2-E2; 11 (6.8%) were G3-P[8]-I1-E1; 12 (7.4%) were G4-P[8]-I1-E1; and 31 (19.1%) were G9-P[8]-I1-E1. Two samples presented mixed genotypes (G1 + G3-P[8]-I1-E1 and G1 + G9-P[9]-I1-E1) and rare combinations, such as G2-P[6]-I2-E2 and G9-P[6]-I2-E2, were detected in six (3.7%) strains. The results suggest a linkage among all four genes. Genotypes G1/G3/G4/G5/G9-P[8] were correlated strongly to I1-E1 genotypes and G2-P[4]/P[6] were correlated to I2-E2 genotypes. Unusual combinations of genes, such as G3-P[9]-I2-E2, G9-P[9]-I1-E2, and G3-P[9]-I3-E3, were observed in 15 (9.3%) strains. The characterization of multiple genes allows a more complete analysis of the rotavirus isolates and provides evidence of natural reassortment of strains.
1996 年至 2006 年间，巴西里约热内卢腹泻患者中检测到的 162 株轮状病毒株通过多基因基因分型进行了分析。通过 RT-PCR 检测对病毒株进行特征分析，以扩增和分型 VP7、VP4、VP6 和 NSP4 编码基因。总体而言，139 株（85.8%）属于常见的 A 组轮状病毒组合：67 株（41.4%）属于基因型 G1-P[8]-I1-E1；18 株（11.1%）为 G2-P[4]-I2-E2；11 株（6.8%）为 G3-P[8]-I1-E1；12 株（7.4%）为 G4-P[8]-I1-E1；31 株（19.1%）为 G9-P[8]-I1-E1。两个样本呈现混合基因型（G1+G3-P[8]-I1-E1 和 G1+G9-P[9]-I1-E1）和罕见组合，如 G2-P[6]-I2-E2 和 G9-P[6]-I2-E2，在 6 株（3.7%）中检测到。结果表明所有四个基因之间存在联系。基因型 G1/G3/G4/G5/G9-P[8]与 I1-E1 基因型密切相关，G2-P[4]/P[6]与 I2-E2 基因型相关。观察到 G3-P[9]-I2-E2、G9-P[9]-I1-E2 和 G3-P[9]-I3-E3 等基因的不寻常组合，在 15 株（9.3%）病毒株中检测到。对多个基因的特征分析可以更全面地分析轮状病毒分离株，并提供了病毒株自然重配的证据。
