Tang Ying, Wang Chang-hai, Huang Di
Dept. of Biosci. and Biotechnol., Dalian Univ. of Technol, Dalian 116024, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2010 Jul;30(7):1956-9.
The contents of eleven mineral elements, including Mg, Fe, K, Ca, Na, Mn, Zn, Cu, Ni, Cd and Cr contents of axenic and natural Dunaliella salina and their culture supernatants in the different period of exponential phase were determined with flame atomic absorption spectrophotometer(AAS). The results show as follows: (1) The contents of Mg, Fe, K, Ca, and Na are between 1 and 10 mg x g(-1). The contents of Zn, Cu, Mn, and Ni are between 0.1 and 1 mg x g(-1). There are little Cd and Cr in the microalgae. (2) The changes in the content of mineral elements of axenic and natural Dunaliella salina during different phases are almost the same. The contents of Mg, Fe, K, Ca, Na, Mn and Cu decreased along with the growth of the microalgae, especially the content of Ca. The contents of Mg, Fe, K, Ca and Na in the culture supernatants keep stable in the culture process and have no distinct difference among axenic and natural Dunaliella salina. But the contents of Cu and Mn in the culture supernatants increased greatly in the middle and end of exponential phase. (3) The contents of Mg, K, Cu, and Ni show no significant differences in axenic and natural microalgae. The contents of Fe, Ca, Na and Zn in the natural microalgae decreased greatly in the middle of exponential phase and were less than in axenic one, but increased at the end of exponential phase and were higher than in axenic one. These results provide reference for further to applying the resource of Dunaliella salina and studying the relationship of microalgae and associated bacteria in the culture.
采用火焰原子吸收分光光度计（AAS）测定了指数生长期不同阶段无菌和天然盐藻及其培养上清液中11种矿物元素（Mg、Fe、K、Ca、Na、Mn、Zn、Cu、Ni、Cd和Cr）的含量。结果如下：（1）Mg、Fe、K、Ca和Na的含量在1至10 mg·g⁻¹之间。Zn、Cu、Mn和Ni的含量在0.1至1 mg·g⁻¹之间。微藻中Cd和Cr含量极少。（2）无菌和天然盐藻在不同阶段矿物元素含量的变化基本相同。Mg、Fe、K、Ca、Na、Mn和Cu的含量随微藻生长而降低，尤其是Ca的含量。培养上清液中Mg、Fe、K、Ca和Na的含量在培养过程中保持稳定，无菌和天然盐藻之间无明显差异。但培养上清液中Cu和Mn的含量在指数生长期中期和末期大幅增加。（3）无菌和天然微藻中Mg、K、Cu和Ni的含量无显著差异。天然微藻中Fe、Ca、Na和Zn的含量在指数生长期中期大幅降低且低于无菌微藻，但在指数生长期末期增加且高于无菌微藻。这些结果为进一步开发利用盐藻资源及研究培养过程中微藻与相关细菌的关系提供了参考。
