Department of Otorhinolaryngology and Medical Research Institute, Pusan National University School of Medicine, Pusan, Korea.
J Otolaryngol Head Neck Surg. 2010 Oct;39(5):600-5.
The major aims of this study were to characterize the varieties of T-cell infiltrates in tissue collected from patients with chronic rhinosinusitis with nasal polyps (CRSwNP), analyze the cytokine profiles of these infiltrating T cells, and determine whether infiltrating T lymphocytes are specific for superantigens (SAGs).
Anterior ethmoid sinus mucosa and polyp tissue were collected from 23 patients with CRSwNP, and control anterior ethmoid sinus mucosa were obtained from 20 patients without CRS. Infiltrating cells were isolated from tissue samples and analyzed using flow cytometry, enzyme-linked immunosorbent assay, reverse transcriptase-polymerase chain reaction, and in vitro T-lymphocyte stimulation with Staphylococcus aureus.
The mean total numbers and proportions of CD3+, CD4+, and CD8+ T cells were significantly higher in the mucosa and polyp tissue of patients with CRSwNP than in the control group. Most infiltrating T cells in patients with CRSwNP were activated CD45RO+ memory T cells. Furthermore, interferon-γ (IFN-γ) expression was significantly higher than interleukin (IL)-10 and IL-4 expression in infiltrating T cells isolated from both the mucosa and the polyp tissue. IFN-γ also showed significantly greater increases in expression compared to IL-4 and IL-10 when isolated T cells were stimulated with SAGs in vitro.
IFN-γ-producing T cells could play an important role in CRSwNP when sinonasal chronic inflammation is induced by SAGs.
本研究的主要目的是描述慢性鼻-鼻窦炎伴鼻息肉（CRSwNP）患者组织中 T 细胞浸润的多样性，分析这些浸润 T 细胞的细胞因子谱，并确定浸润性 T 淋巴细胞是否针对超抗原（SAGs）。
从前鼻嵴筛窦黏膜和息肉组织中收集 23 例 CRSwNP 患者的组织样本，从前鼻嵴筛窦黏膜中收集 20 例无 CRS 的患者作为对照。从组织样本中分离浸润细胞，并用流式细胞术、酶联免疫吸附试验、逆转录-聚合酶链反应和金黄色葡萄球菌体外 T 淋巴细胞刺激进行分析。
CRSwNP 患者的黏膜和息肉组织中 CD3+、CD4+和 CD8+T 细胞的总数和比例明显高于对照组。CRSwNP 患者浸润的 T 细胞大多数为活化的 CD45RO+记忆 T 细胞。此外，与 IL-10 和 IL-4 相比，IFN-γ在从黏膜和息肉组织中分离的浸润 T 细胞中的表达显著更高。当体外刺激 T 细胞时，IFN-γ的表达与 IL-4 和 IL-10 相比也显著增加。
当 SAGs 引起鼻-鼻窦慢性炎症时，产生 IFN-γ的 T 细胞可能在 CRSwNP 中发挥重要作用。
