Molecular Biology Division, IBR (Instituto de Biología Molecular y Celular de Rosario), Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, Rosario, Argentina.
J Plant Physiol. 2011 Mar 1;168(4):382-91. doi: 10.1016/j.jplph.2010.07.014. Epub 2010 Sep 9.
Plants are continuously exposed to pathogen challenge. The most common defense response to pathogenic microorganisms is the nonhost response, which is usually accompanied by transcriptional changes. In order to identify genes involved in nonhost resistance, we evaluated the tobacco transcriptome profile after infection with Xanthomonas axonopodis pv. citri (Xac), a nonhost phytopathogenic bacterium. cDNA-amplified fragment length polymorphism was used to identify differentially expressed transcripts in tobacco leaves infected with Xac at 2, 8 and 24h post-inoculation. From a total of 2087 transcript-derived fragments (TDFs) screened (approximately 20% of the tobacco transcriptome), 316 TDFs showed differential expression. Based on sequence similarities, 82 differential TDFs were identified and assigned to different functional categories: 56 displayed homology to genes with known functions, 12 to proteins with unknown functions and 14 did not have a match. Real-time PCR was carried out with selected transcripts to confirm the expression pattern obtained. The results reveal novel genes associated with nonhost resistance in plant-pathogen interaction in tobacco. These novel genes could be included in future strategies of molecular breeding for nonhost disease resistance.
植物不断受到病原体的挑战。植物对病原微生物最常见的防御反应是非寄主反应，通常伴随着转录变化。为了鉴定参与非寄主抗性的基因，我们评估了感染非寄主植物病原细菌柑橘溃疡病菌（Xac）后烟草的转录组图谱。利用 cDNA 扩增片段长度多态性（cDNA-AFLP）技术，在接种后 2、8 和 24 小时检测到烟草叶片中差异表达的转录本。在总共筛选出的 2087 个转录物衍生片段（TDF）中（约占烟草转录组的 20%），有 316 个 TDF 表现出差异表达。基于序列相似性，鉴定出 82 个差异 TDF，并将其分配到不同的功能类别：56 个显示与具有已知功能的基因同源，12 个与具有未知功能的蛋白质同源，14 个没有匹配。用选定的转录本进行实时 PCR 以确认获得的表达模式。结果揭示了与烟草中植物-病原体互作中非寄主抗性相关的新基因。这些新基因可以包含在未来非寄主抗病性的分子育种策略中。
