Zamaraeva T V, Insarova I D, Mazurov V I
Vopr Med Khim. 1978 May-Jun;24(3):419-23.
A method, described for obtaining of 14C-collagen from chicken embryo tendons, permitted to isolate the highly purified native substrate with high specific radioactivity applied to estimation of animal collagenases activity. The method is relatively simple, reproducible, does not require large amount of labelled amino acids; it shortens the course of protein purification.
一种从鸡胚肌腱中获取14C标记胶原蛋白的方法,可用于分离具有高比放射性的高度纯化的天然底物,以评估动物胶原酶的活性。该方法相对简单、可重复,不需要大量标记氨基酸,缩短了蛋白质纯化过程。