Simon Carola, Daniel Rolf
Institute of Microbiology and Genetics, Georg August University Göttingen, Göttingen, Germany.
Methods Mol Biol. 2010;668:39-50. doi: 10.1007/978-1-60761-823-2_2.
The vast majority of the Earth's biological diversity is hidden in uncultured and yet uncharacterized microbial genomes. The construction of metagenomic libraries is a cultivation-independent molecular approach to assess this unexplored genetic reservoir. In the last few years, a high number of novel biocatalysts have been identified by function-based or sequence-based screening of metagenomic libraries. Here, we describe detailed protocols for the construction of metagenomic small-insert and large-insert libraries in plasmids and fosmids, respectively, from environmental DNA.
地球上绝大多数生物多样性隐藏于未培养且未表征的微生物基因组中。宏基因组文库的构建是一种不依赖培养的分子方法，用于评估这一未开发的基因库。在过去几年中，通过基于功能或基于序列的宏基因组文库筛选，已鉴定出大量新型生物催化剂。在此，我们分别描述了从环境DNA构建质粒和fosmid宏基因组小插入和大插入文库的详细方案。
