Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA.
Curr Opin Neurobiol. 2010 Oct;20(5):667-75. doi: 10.1016/j.conb.2010.08.002. Epub 2010 Sep 15.
Reconstructing neuronal circuits at the level of synapses is a central problem in neuroscience, and the focus of the nascent field of connectomics. Previously used to reconstruct the C. elegans wiring diagram, serial-section transmission electron microscopy (ssTEM) is a proven technique for the task. However, to reconstruct more complex circuits, ssTEM will require the automation of image processing. We review progress in the processing of electron microscopy images and, in particular, a semi-automated reconstruction pipeline deployed at Janelia Farm. Drosophila circuits underlying identified behaviors are being reconstructed in the pipeline with the goal of generating a complete Drosophila connectome.
重建突触水平的神经元回路是神经科学的一个核心问题，也是连接组学这一新兴领域的重点。以前曾用于重建秀丽隐杆线虫的连接图，连续切片透射电子显微镜（ssTEM）是一项经过验证的任务技术。然而，要重建更复杂的电路，ssTEM 将需要图像处理的自动化。我们回顾了电子显微镜图像处理方面的进展，特别是在 Janelia Farm 部署的半自动重建管道。该管道正在重建果蝇识别行为背后的电路，目标是生成一个完整的果蝇连接组。
