Paczoska-Eliasiewicz H, Popek W, Rzasa J, Witkowska B
Department of Animal Physiology, Agricultural University, Kraków, Poland.
Folia Biol (Krakow). 1990;38(1-4):27-33.
The purpose of the present study was to demonstrate visually and localize the presence of serotonin (5-HT) in the ovary and oviduct of the domestic hen using a histochemical Falck-Hillarp method. Experiments were carried out on White Leghorn laying hens with no egg in the shell gland. The specific yellow fluorescence, indicating the presence of 5-HT, was found both in the ovary and all examined oviductal parts. The strongest fluorescence was present in the ovarian stroma containing small follicles with a diameter under 4 mm. In the wall of the largest preovulatory follicle a very strong fluorescence was located mainly in the theca layer. In the oviductal parts, the intensity of 5-HT fluorescence in the infundibulum and magnum was fairly strong, whereas in the isthmus and shell gland it was weak. Fluorescence seen in the infundibulum, magnum, and isthmus was primarily localized along the luminal borders of the fold surface epithelium. In the shell gland 5-HT fluorescence was found within the uterine folds, especially in the tubular glands. Moreover, the presence of an egg in the definite oviductal segment (infundibulum or isthmus) increased the intensity of yellow fluorescence in this part.
本研究的目的是使用福尔克 - 希拉尔普组织化学方法，直观地展示并定位家鸡卵巢和输卵管中5 - 羟色胺（5 - HT）的存在。实验在子宫腺中无蛋的白来航产蛋母鸡身上进行。表明5 - HT存在的特定黄色荧光在卵巢和所有检查的输卵管部位均被发现。最强的荧光出现在含有直径小于4毫米小卵泡的卵巢基质中。在最大的排卵前卵泡壁中，非常强的荧光主要位于卵泡膜层。在输卵管各部位，漏斗部和蛋白分泌部的5 - HT荧光强度相当强，而在峡部和子宫腺中则较弱。在漏斗部、蛋白分泌部和峡部看到的荧光主要沿褶皱表面上皮的管腔边界定位。在子宫腺中，5 - HT荧光在子宫皱襞内被发现，尤其是在管状腺中。此外，在特定输卵管段（漏斗部或峡部）中有蛋存在会增加该部位黄色荧光的强度。
