Ighodaro O M, Ebuehi O A T
Department of Biochemistry, Lead City University, Ibadan, Nigeria.
Nig Q J Hosp Med. 2009 Apr-Jun;19(2):106-9.
Ocimum gratissimum is a vegetable plant of wide nutritional and medicinal aŕpplications in Nigeria and in some other parts of the world.
The effect of oral administration of aqueous leaf extract of O. gratissimum on the activities of plasma and hepatic anti-oxidant enzymes in rats was investigated.
Two different doses of aqueous leaf extract of O. gratissimum (0.2 mg/g and 0.4 mg/kg body weight) were separately administered orally daily into rats for 4 weeks. The rats were sacrificed after 30 days of administration, blood was collected and the liver excised. The liver was homogenized, while plasma and hepatic anti-oxidant enzymes, namely catalase (CAT), superoxide dismutase (SOD) and glutathione -S- transferase (GST) specific activities were assayed.
Results of the study showed a significant (p < 0.05) increase in the specific activity of both plasma and hepatic CAT and SOD in a dose dependent pattern. However, the effect of the leaf extract on GST specific activity was not significant (p > 0.05) as compared to the control. There were no significant changes in the plasma and liver protein concentrations in rats administered the extracts as compared to the control.
Data of the study suggest that the oral administration of the aqueous extract of O. gratissimum may impair naturally generated oxidant/toxicant activity and thereby enhance specific activities of hepatic antioxidant enzymes in rats.
在尼日利亚及世界其他一些地区，九层塔是一种具有广泛营养和药用价值的蔬菜植物。
研究口服九层塔叶水提取物对大鼠血浆和肝脏抗氧化酶活性的影响。
将两种不同剂量的九层塔叶水提取物（0.2毫克/克和0.4毫克/千克体重）分别每日口服给予大鼠，持续4周。给药30天后处死大鼠，采集血液并切除肝脏。将肝脏匀浆，同时测定血浆和肝脏抗氧化酶，即过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和谷胱甘肽-S-转移酶（GST）的比活性。
研究结果显示，血浆和肝脏中CAT和SOD的比活性均呈剂量依赖性显著增加（p<0.05）。然而，与对照组相比，叶提取物对GST比活性的影响不显著（p>0.05）。与对照组相比，给予提取物的大鼠血浆和肝脏蛋白质浓度无显著变化。
该研究数据表明，口服九层塔水提取物可能会损害自然产生的氧化剂/毒物活性，从而提高大鼠肝脏抗氧化酶的比活性。
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