Institute of Microbiology and Immunology, Chung Shan Medical University, Taichung 40201, Taiwan.
Evid Based Complement Alternat Med. 2012;2012:736752. doi: 10.1155/2012/736752. Epub 2012 Jun 27.
Ocimum gratissimum (OG) is known as a food spice and traditional herb, which has been recommended for the treatment of various diseases. To investigate the hepatoprotective effect of OG aqueous extract (OGAE), male Wistar rats challenged by carbon tetrachloride (CCl(4)) were used as the animal model of chronic hepatic injury. Significantly increased serum catalase and DPPH levels were detected in CCl(4)-administrated rats that were treated with OGAE or silymarin as compared to those rats that were treated with saline or CCl(4). In contrast, significantly decreased stress proteins including HSP70 and iNOS were observed in livers of CCl(4)-administrated rats that were treated with OGAE or sylimarin as compared to those rats that were treated with saline or CCl(4). Moreover, significant decreases of MMP-9/MMP-2 ratio, uPA, phosphorylated ERK (p-ERK) and NF-κB (p-P65) were detected in livers of CCl(4)-administrated rats that were treated with OGAE or sylimarin as compared to those rats that were treated with saline or CCl(4). These findings imply that OGAE can efficiently inhibit CCl(4)-induced liver injuries in rats and may therefore be a potential food or herb for preventing liver injuries.
奥克图姆 gratissimum（OG）被称为食品香料和传统草药，已被推荐用于治疗各种疾病。为了研究 OG 水提取物（OGAE）的保肝作用，雄性 Wistar 大鼠用四氯化碳（CCl（4））作为慢性肝损伤的动物模型。与用盐水或 CCl（4）处理的大鼠相比，用 OGAE 或水飞蓟素治疗的 CCl（4）处理的大鼠血清过氧化氢酶和 DPPH 水平显著升高。相反，与用盐水或 CCl（4）处理的大鼠相比，用 OGAE 或水飞蓟素治疗的 CCl（4）处理的大鼠肝脏中的应激蛋白 HSP70 和 iNOS 明显降低。此外，与用盐水或 CCl（4）处理的大鼠相比，用 OGAE 或水飞蓟素治疗的 CCl（4）处理的大鼠肝脏中的 MMP-9/MMP-2 比值、uPA、磷酸化 ERK（p-ERK）和 NF-κB（p-P65）显著降低。这些发现表明，OGAE 能有效抑制 CCl（4）诱导的大鼠肝损伤，因此可能是预防肝损伤的潜在食品或草药。
