Department of Emergency Medicine and Critical Care, Saitama Medical Centre, 1981 Kamoda, Kawagoe, Saitama 350-8558, Japan.
J Clin Biochem Nutr. 2010 Sep;47(2):107-10. doi: 10.3164/jcbn.10-22. Epub 2010 Jun 17.
Past studies have suggested that some carbon monoxide (CO) moves from blood haemoglobin to tissue cells and that mitochondrial cytochrome c oxidase oxidizes CO to carbon dioxide (CO(2)). However, no study has demonstrated this redistribution and oxidization of CO under physiological conditions. The objective of this study was to trace the redistribution and oxidization of CO in the human body by detecting (13)CO(2) production after the inhalation of (13)CO. In Experiment 1, we asked a healthy subject to inhale 50 ppm (13)CO gas. In Experiment 2, we circulated heparinized human blood in a cardio-pulmonary bypass circuit and supplied 50 ppm (13)CO gas to the oxygenator. We sequentially sampled exhaled and output gases and measured the (13)CO(2)/(12)CO(2) ratios. In Experiment 1, the exhaled (13)CO(2)/(12)CO(2) ratio increased significantly between 4 to 31 h of (13)CO inhalation. In Experiment 2, the output (13)CO(2)/(12)CO(2) ratio showed no significant increase within 36 h of (13)CO input. Experiment 1 demonstrated the oxidization of CO in the human body under physiological conditions. Experiment 2 confirmed that oxidization does not occur in the circulating blood and indicated the redistribution of CO from blood carboxyhaemoglobin to tissue cells.
过去的研究表明，一部分一氧化碳（CO）从血液血红蛋白转移到组织细胞，线粒体细胞色素 c 氧化酶将 CO 氧化为二氧化碳（CO(2)）。然而，尚无研究在生理条件下证明 CO 的这种再分布和氧化。本研究旨在通过检测吸入（13）CO 后（13）CO(2)的产生来追踪人体内 CO 的再分布和氧化。在实验 1 中，我们让一名健康受试者吸入 50ppm（13）CO 气体。在实验 2 中，我们将肝素化的人体血液在心肺旁路回路中循环，并向氧合器供应 50ppm（13）CO 气体。我们依次对呼出气体和输出气体进行采样，并测量（13）CO(2)/(12)CO(2)比值。在实验 1 中，吸入（13）CO 后 4 至 31 小时，呼出（13）CO(2)/(12)CO(2)比值显著增加。在实验 2 中，输入（13）CO 36 小时内输出（13）CO(2)/(12)CO(2)比值无明显增加。实验 1 证明了 CO 在生理条件下在人体内的氧化。实验 2 证实了循环血液中不会发生氧化，并表明 CO 从血液碳氧血红蛋白向组织细胞的再分布。
