Jacob Richard J
Matrix Science Inc., Boston, MA, USA.
Methods Mol Biol. 2010;658:61-91. doi: 10.1007/978-1-60761-780-8_4.
Mass spectrometry instrumentation has continued to develop rapidly in the last two decades, enabled in part by advances in microelectronic hardware controllers and computerized control and data acquisition systems. The wealth and complexity of data produced by a modern instrument is such that the data can no longer be analyzed manually. Computerized data analysis has become de rigueur and the bioinformatics field has expanded to provide software applications for all aspects of the data analysis needed by LC-MS/MS. The bioinformatics field is evolving rapidly and software applications are continually being improved or replaced for existing applications as well as developed to support new types of experiments and analysis enabled by modern instrumentation. Entire books have been written on MS data analysis in proteomics but this review will be necessarily brief. In this chapter we will review the bioinformatics software applications available for different LC-MS/MS analysis tasks.
在过去二十年中，质谱仪器持续快速发展，这在一定程度上得益于微电子硬件控制器以及计算机化控制和数据采集系统的进步。现代仪器产生的数据量巨大且复杂，已无法手动分析。计算机化数据分析已成为必需，生物信息学领域不断扩展，以提供用于液相色谱 - 串联质谱（LC-MS/MS）所需数据分析各个方面的软件应用程序。生物信息学领域发展迅速，软件应用程序不断改进或取代现有应用程序，同时也在开发以支持现代仪器实现的新型实验和分析。关于蛋白质组学中质谱数据分析的书籍已有很多，但本综述将必然简短。在本章中，我们将回顾可用于不同LC-MS/MS分析任务的生物信息学软件应用程序。
